151 211

Cited 34 times in

Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair

DC FieldValueLanguage
dc.contributor.author윤영섭-
dc.contributor.author이지윤-
dc.date.accessioned2016-02-04T11:29:42Z-
dc.date.available2016-02-04T11:29:42Z-
dc.date.issued2015-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/140550-
dc.description.abstractHuman pluripotent stem cells (hPSCs) have emerged as an important source for cell therapy. However, to date, no studies demonstrated generation of purified hPSC-derived lymphatic endothelial cells (LECs) and tested their therapeutic potential in disease models. Here we sought to differentiate hPSCs into the LEC lineage, purify them with LEC markers, and evaluate their therapeutic effects. We found that an OP9-assisted culture system reinforced by addition of VEGF-A, VEGF-C, and EGF most efficiently generated LECs, which were then isolated via FACS-sorting with LYVE-1 and PODOPLANIN. These hPSC-derived LYVE-1(+)PODOPLANIN(+)cells showed a pure committed LEC phenotype, formed new lymphatic vessels, and expressed lymphangiogenic factors at high levels. These hPSC-derived LECs enhanced wound healing through lymphangiogenesis and lymphvasculogenesis. Here we report, for the first time, that LECs can be selectively isolated from differentiating hPSCs, and that these cells are potent for lymphatic vessel formation in vivo and wound healing. This system and the purified hPSC-derived LECs can serve as a new platform for studying LEC development as well as for cell therapy.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfSCIENTIFIC REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Differentiation*-
dc.subject.MESHCell- and Tissue-Based Therapy*-
dc.subject.MESHEndothelial Cells/cytology-
dc.subject.MESHEndothelial Cells/metabolism*-
dc.subject.MESHEndothelial Cells/transplantation-
dc.subject.MESHEpidermal Growth Factor/pharmacology-
dc.subject.MESHHeterografts-
dc.subject.MESHHumans-
dc.subject.MESHLymphangiogenesis*-
dc.subject.MESHMice-
dc.subject.MESHMice, Nude-
dc.subject.MESHPluripotent Stem Cells-
dc.subject.MESHVascular Endothelial Growth Factor A/pharmacology-
dc.subject.MESHVascular Endothelial Growth Factor C/pharmacology-
dc.subject.MESHWound Healing*-
dc.titleGeneration of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Life Science (의생명과학부)-
dc.contributor.googleauthorShin-Jeong Lee-
dc.contributor.googleauthorChangwon Park-
dc.contributor.googleauthorJi Yoon Lee-
dc.contributor.googleauthorSangsung Kim-
dc.contributor.googleauthorPil Jae Kwon-
dc.contributor.googleauthorWoansang Kim-
dc.contributor.googleauthorYong Heui Jeon-
dc.contributor.googleauthorEugine Lee-
dc.contributor.googleauthorYoung-sup Yoon-
dc.identifier.doi10.1038/srep11019-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02579-
dc.contributor.localIdA03206-
dc.relation.journalcodeJ02646-
dc.identifier.eissn2045-2322-
dc.identifier.pmid26066093-
dc.contributor.alternativeNameYoon, Young Sup-
dc.contributor.alternativeNameLee, Ji Yoon-
dc.contributor.affiliatedAuthorYoon, Young Sup-
dc.contributor.affiliatedAuthorLee, Ji Yoon-
dc.rights.accessRightsfree-
dc.citation.volume5-
dc.citation.startPage11019-
dc.identifier.bibliographicCitationSCIENTIFIC REPORTS, Vol.5 : 11019, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.