Cited 13 times in
Regulation of IGFBP-2 expression during fasting
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김미영 | - |
dc.contributor.author | 안용호 | - |
dc.date.accessioned | 2016-02-04T11:22:03Z | - |
dc.date.available | 2016-02-04T11:22:03Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140267 | - |
dc.description.abstract | Insulin-like growth factor (IGF)-binding protein-2 (IGFBP-2), one of the most abundant circulating IGFBPs, is known to attenuate the biological action of IGF-1. Although the effect of IGFBP-2 in preventing metabolic disorders is well known, its regulatory mechanism remains unclear. In the present study, we demonstrated the transcriptional regulation of the Igfbp-2 gene by peroxisome-proliferator-activated receptor (PPAR) α in the liver. During fasting, both Igfbp-2 and PPARα expression levels were increased. Wy14643, a selective PPARα agonist, significantly induced Igfbp-2 gene expression in primary cultured hepatocytes. However, Igfbp-2 gene expression in Pparα null mice was not affected by fasting or Wy14643. In addition, through transient transfection and chromatin immunoprecipitation assay in fasted livers, we determined that PPARα bound to the putative PPAR-responsive element between -511 bp and -499 bp on the Igfbp-2 gene promoter, indicating that the Igfbp-2 gene transcription is activated directly by PPARα. To explore the role of PPARα in IGF-1 signalling, we treated primary cultured hepatocytes with Wy14643 and observed a decrease in the number of IGF-1 receptors (IGF-1Rs) and in Akt phosphorylation. No inhibition was observed in the hepatocytes isolated from Pparα null mice. These results suggest that PPARα controls IGF-1 signalling through the up-regulation of hepatic Igfbp-2 transcription during fasting and Wy14643 treatment. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 453~460 | - |
dc.relation.isPartOf | BIOCHEMICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Fasting/metabolism* | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Hepatocytes/drug effects | - |
dc.subject.MESH | Hepatocytes/metabolism | - |
dc.subject.MESH | Insulin-Like Growth Factor Binding Protein 2/genetics* | - |
dc.subject.MESH | Insulin-Like Growth Factor I/metabolism | - |
dc.subject.MESH | Liver/drug effects | - |
dc.subject.MESH | Liver/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | PPAR alpha/deficiency | - |
dc.subject.MESH | PPAR alpha/genetics | - |
dc.subject.MESH | PPAR gamma/agonists | - |
dc.subject.MESH | Peroxisome Proliferators/pharmacology | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Proto-Oncogene Proteins c-akt/metabolism | - |
dc.subject.MESH | Pyrimidines/pharmacology | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Thiazolidinediones/pharmacology | - |
dc.subject.MESH | Up-Regulation/drug effects | - |
dc.title | Regulation of IGFBP-2 expression during fasting | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Hye Suk Kang | - |
dc.contributor.googleauthor | Mi-Young Kim | - |
dc.contributor.googleauthor | Seung-Jae Kim | - |
dc.contributor.googleauthor | Jae-Ho Lee | - |
dc.contributor.googleauthor | Yong-Deuk Kim | - |
dc.contributor.googleauthor | Young-Kyo Seo | - |
dc.contributor.googleauthor | Jae-Hoon Bae | - |
dc.contributor.googleauthor | Goo-Taeg Oh | - |
dc.contributor.googleauthor | Dae-Kyu Song | - |
dc.contributor.googleauthor | Yong-Ho Ahn | - |
dc.contributor.googleauthor | Seung-Soon Im | - |
dc.identifier.doi | 10.1042/BJ20141248 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00446 | - |
dc.contributor.localId | A02249 | - |
dc.relation.journalcode | J00282 | - |
dc.identifier.eissn | 1470-8728 | - |
dc.identifier.pmid | 25695641 | - |
dc.subject.keyword | fasting | - |
dc.subject.keyword | gene expression | - |
dc.subject.keyword | insulin-like growth factor-1 (IGF-1) | - |
dc.subject.keyword | insulin-like growth factor-binding protein 2 (IGFBP-2) | - |
dc.subject.keyword | liver | - |
dc.subject.keyword | peroxisome-proliferator-activated receptor α (PPARα) | - |
dc.contributor.alternativeName | Kim, Mi Young | - |
dc.contributor.alternativeName | Ahn, Yong Ho | - |
dc.contributor.affiliatedAuthor | Kim, Mi Young | - |
dc.contributor.affiliatedAuthor | Ahn, Yong Ho | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 467 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 453 | - |
dc.citation.endPage | 460 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL JOURNAL, Vol.467(3) : 453-460, 2015 | - |
dc.identifier.rimsid | 50389 | - |
dc.type.rims | ART | - |
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