0 365

Cited 9 times in

Pyruvate metabolism: A therapeutic opportunity in radiation-induced skin injury

Authors
 Hyun Yoo  ;  Jeong Wook Kang  ;  Dong Won Lee  ;  Sang Ho Oh  ;  Yun-Sil Lee  ;  Eun-Jung Lee  ;  Jaeho Cho 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.460(3) : 504-510, 2015 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2015
MeSH
Animals ; Cytokines/genetics ; Gene Expression Regulation/genetics ; Mice ; NIH 3T3 Cells ; Protein-Serine-Threonine Kinases/genetics ; Pyruvates/metabolism* ; Radiation Injuries, Experimental/metabolism ; Radiation Injuries, Experimental/therapy* ; Real-Time Polymerase Chain Reaction ; Skin/injuries ; Skin/radiation effects*
Keywords
Pyruvate dehydrogenase kinase ; Radiation ; Skin injury
Abstract
Ionizing radiation is used to treat a range of cancers. Despite recent technological progress, radiation therapy can damage the skin at the administration site. The specific molecular mechanisms involved in this effect have not been fully characterized. In this study, the effects of pyruvate, on radiation-induced skin injury were investigated, including the role of the pyruvate dehydrogenase kinase 2 (PDK2) signaling pathway. Next generation sequencing (NGS) identified a wide range of gene expression differences between the control and irradiated mice, including reduced expression of PDK2. This was confirmed using Q-PCR. Cell culture studies demonstrated that PDK2 overexpression and a high cellular pyruvate concentration inhibited radiation-induced cytokine expression. Immunohistochemical studies demonstrated radiation-induced skin thickening and gene expression changes. Oral pyruvate treatment markedly downregulated radiation-induced changes in skin thickness and inflammatory cytokine expression. These findings indicated that regulation of the pyruvate metabolic pathway could provide an effective approach to the control of radiation-induced skin damage.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X15005069
DOI
10.1016/j.bbrc.2015.03.060
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Oh, Sang Ho(오상호) ORCID logo https://orcid.org/0000-0002-4477-1400
Lee, Dong Won(이동원) ORCID logo https://orcid.org/0000-0003-0046-3139
Lee, Eun Jung(이은정)
Cho, Jae Ho(조재호) ORCID logo https://orcid.org/0000-0001-9966-5157
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140107
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links