Cited 12 times in
Immune modulatory effect of thalidomide on T cells
DC Field | Value | Language |
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dc.contributor.author | 김명수 | - |
dc.contributor.author | 김범석 | - |
dc.contributor.author | 김유선 | - |
dc.contributor.author | 이재근 | - |
dc.contributor.author | 허규하 | - |
dc.date.accessioned | 2016-02-04T11:12:44Z | - |
dc.date.available | 2016-02-04T11:12:44Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139915 | - |
dc.description.abstract | BACKGROUND: Thalidomide was originally used to alleviate morning sickness in pregnant women, but was banned due to severe adverse effects. Since the discovery of its anticancer and anti-inflammatory properties, it has regained research interest. However, its mechanism of action is still unknown. Therefore, we examined the effects of thalidomide on effector T (Teff) and regulatory T (Treg) cells in splenocytes of mice. METHODS: Splenic CD4(+), CD44(low), and CD62L(high) T lymphocytes (Tnaives) isolated from C57BL/6 mice were cultured for T-cell proliferation and Treg conversion. For T-cell proliferation, naive T cells (Tnaives) were cultured for 72 hours with anti-CD3 and anti-CD28 antibodies, and carboxyfluorescein succinimidyl ester (CFSE) labeling method was used. For Treg conversion, Tnaives were cultured for 72 hours with transforming growth factor-β1 (TGF-β1) and interleukin-2 (IL-2). Naïve T cells were plated at 1.5 × 10(5) cells on 96-well plates with 0, 1, 10, 50, or 100 μmol/L thalidomide. All samples were analyzed by flow cytometry after staining with CFSE, APC-conjugated anti-mouse CD4, and FITC-conjugated anti-mouse FoxP3. RESULTS: Thalidomide significantly decreased the proliferation of CD4(+) Teffs in a dose-dependent manner (P < .01). In contrast, conversion to CD4(+)FoxP3(+) Tregs tended to increase by thalidomide treatment, although the increase was not statistically significant. CONCLUSION: These findings suggest that thalidomide may have an immune modulatory effect by selectively suppressing CD4(+) Teff proliferation. Further studies will be needed to elucidate the underlying signaling pathway. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 787~790 | - |
dc.relation.isPartOf | TRANSPLANTATION PROCEEDINGS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | CD4-Positive T-Lymphocytes/drug effects | - |
dc.subject.MESH | CD4-Positive T-Lymphocytes/immunology | - |
dc.subject.MESH | Cell Proliferation/drug effects* | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Immunosuppressive Agents/pharmacology* | - |
dc.subject.MESH | Lymphocyte Activation/drug effects* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Spleen/cytology | - |
dc.subject.MESH | T-Lymphocytes, Regulatory/drug effects* | - |
dc.subject.MESH | T-Lymphocytes, Regulatory/immunology | - |
dc.subject.MESH | Thalidomide/pharmacology* | - |
dc.title | Immune modulatory effect of thalidomide on T cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | B.S. Kim | - |
dc.contributor.googleauthor | J.Y. Kim | - |
dc.contributor.googleauthor | J.G. Lee | - |
dc.contributor.googleauthor | Y. Cho | - |
dc.contributor.googleauthor | K.H. Huh | - |
dc.contributor.googleauthor | M.S. Kim | - |
dc.contributor.googleauthor | Y.S. Kim | - |
dc.identifier.doi | 10.1016/j.transproceed.2014.12.038 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00488 | - |
dc.contributor.localId | A00785 | - |
dc.contributor.localId | A03068 | - |
dc.contributor.localId | A04344 | - |
dc.contributor.localId | A00424 | - |
dc.relation.journalcode | J02755 | - |
dc.identifier.eissn | 1873-2623 | - |
dc.identifier.pmid | 25891732 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0041134515001190 | - |
dc.contributor.alternativeName | Kim, Myoung Soo | - |
dc.contributor.alternativeName | Kim, Beom Seok | - |
dc.contributor.alternativeName | Kim, Yu Seun | - |
dc.contributor.alternativeName | Lee, Jae Geun | - |
dc.contributor.alternativeName | Huh, Kyu Ha | - |
dc.contributor.affiliatedAuthor | Kim, Beom Seok | - |
dc.contributor.affiliatedAuthor | Kim, Yu Seun | - |
dc.contributor.affiliatedAuthor | Lee, Jae Geun | - |
dc.contributor.affiliatedAuthor | Huh, Kyu Ha | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 47 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 787 | - |
dc.citation.endPage | 790 | - |
dc.identifier.bibliographicCitation | TRANSPLANTATION PROCEEDINGS, Vol.47(3) : 787-790, 2015 | - |
dc.identifier.rimsid | 48375 | - |
dc.type.rims | ART | - |
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