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Comparative study of decalcification versus nondecalcification for histological evaluation of one-wall periodontal intrabony defects in dogs

DC Field Value Language
dc.contributor.author박상현-
dc.contributor.author박영범-
dc.contributor.author최현민-
dc.date.accessioned2016-02-04T11:09:14Z-
dc.date.available2016-02-04T11:09:14Z-
dc.date.issued2015-
dc.identifier.issn1059-910X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/139785-
dc.description.abstractBiological reactions between biomaterials and surrounding tissues, analyzed by histology, may be important predictors of clinical healing pattern and selection of slide preparation techniques requires a careful consideration regarding sample properties. In this study, we compared histology of bone specimens prepared with or without decalcification and performed histological and histomorphometrical assessments. For the histological evaluation, one-wall intrabony defects were created around the mandibular molars of six adult dogs, filled with biphasic calcium phosphate, synthetic bone graft material/recombinant human bone morphogenic protein-2, and healing pattern was histologically evaluated at 4 and 12 weeks. New bone formation in 5 × 4 × 4 mm defects and the length of new cementum, connective tissue attachments around the teeth and number of osteoclasts were measured by histomorphometric analysis. After decalcification, new cementum was easily observed and was significantly increased at week 4. In nondecalcified samples, significantly increased connective tissue attachments were seen at week 12. After 12 weeks, the number of countable multinucleated osteoclasts was significantly increased by 62% in nondecalcified versus calcified tissue sections (P = 0.030). Histomorphometric results may be significantly affected by histological preparation method and therefore, selecting the most appropriate histological preparation method is essential for reliable diagnosis and evaluation of bony samples in studies analyzing tissue regeneration.-
dc.description.statementOfResponsibilityopen-
dc.format.extent94~104-
dc.relation.isPartOfMICROSCOPY RESEARCH AND TECHNIQUE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCalcification, Physiologic-
dc.subject.MESHDogs-
dc.subject.MESHGuided Tissue Regeneration/methods-
dc.subject.MESHHumans-
dc.subject.MESHOsteogenesis/physiology-
dc.subject.MESHPeriodontal Diseases/therapy*-
dc.titleComparative study of decalcification versus nondecalcification for histological evaluation of one-wall periodontal intrabony defects in dogs-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentOral Science Research Center (구강과학연구소)-
dc.contributor.googleauthorSang-Hyun Park-
dc.contributor.googleauthorHyunMin Choi-
dc.contributor.googleauthorJin-Soo Han-
dc.contributor.googleauthorYoung-Bum Park-
dc.identifier.doi10.1002/jemt.22449-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01567-
dc.contributor.localIdA04208-
dc.contributor.localIdA01492-
dc.relation.journalcodeJ02233-
dc.identifier.eissn1097-0029-
dc.identifier.pmid25331781-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jemt.22449/abstract-
dc.subject.keyworddecalcified-
dc.subject.keywordguided tissue regeneration-
dc.subject.keywordhistology-
dc.subject.keywordhistomorphometry-
dc.subject.keywordnondecalcified-
dc.contributor.alternativeNamePark, Sang Hyun-
dc.contributor.alternativeNamePark, Young Bum-
dc.contributor.alternativeNameChoi, Hyun Min-
dc.contributor.affiliatedAuthorPark, Young Bum-
dc.contributor.affiliatedAuthorChoi, Hyun Min-
dc.contributor.affiliatedAuthorPark, Sang Hyun-
dc.rights.accessRightsnot free-
dc.citation.volume78-
dc.citation.number1-
dc.citation.startPage94-
dc.citation.endPage104-
dc.identifier.bibliographicCitationMICROSCOPY RESEARCH AND TECHNIQUE, Vol.78(1) : 94-104, 2015-
dc.identifier.rimsid53821-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Prosthodontics (보철과학교실) > 1. Journal Papers

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