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Biologic response of degenerative living human nucleus pulposus cells to treatment with cytokines

DC Field Value Language
dc.contributor.author구성욱-
dc.contributor.author김근수-
dc.contributor.author김긍년-
dc.contributor.author박정윤-
dc.contributor.author조용은-
dc.contributor.author진동규-
dc.date.accessioned2016-02-04T11:02:28Z-
dc.date.available2016-02-04T11:02:28Z-
dc.date.issued2015-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/139530-
dc.description.abstractPURPOSE: To investigate the molecular responses of various genes and proteins related to disc degeneration upon treatment with cytokines that affect disc-cell proliferation and phenotype in living human intervertebral discs (IVDs). Responsiveness to these cytokines according to the degree of disc degeneration was also evaluated. MATERIALS AND METHODS: The disc specimens were classified into two groups: group 1 (6 patients) showed mild degeneration of IVDs and group 2 (6 patients) exhibited severe degeneration of IVDs. Gene expression was analyzed after treatment with four cytokines: recombinant human bone morphogenic protein (rhBMP-2), transforming growth factor-β (TGF-β), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α). Molecular responses were assessed after exposure of cells from the IVD specimens to these cytokines via real-time polymerase chain reaction and immunofluorescence staining. RESULTS: mRNA gene expression was significantly greater for aggrecan, type I collagen, type II collagen, alkaline phosphatase, osteocalcin, and Sox9 in group 1 than mRNA gene expression in group 2, when the samples were not treated with cytokines. Analysis of mRNA levels for these molecules after morphogen treatment revealed significant increases in both groups, which were much higher in group 1 than in group 2. The average number of IVD cells that were immunofluorescence stained positive for alkaline phosphatase increased after treatment with rhBMP-2 and TGF-β in group 1. CONCLUSION: The biologic responsiveness to treatment of rhBMP-2, TGF-β, TNF-α, and IL-1β in the degenerative living human IVD can be different according to the degree of degeneration of the IVD.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfYONSEI MEDICAL JOURNAL-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAggrecans/genetics-
dc.subject.MESHAggrecans/metabolism-
dc.subject.MESHAlkaline Phosphatase/genetics-
dc.subject.MESHAlkaline Phosphatase/metabolism-
dc.subject.MESHBiological Products/pharmacology-
dc.subject.MESHBiological Products/therapeutic use*-
dc.subject.MESHBone Morphogenetic Protein 2/pharmacology-
dc.subject.MESHBone Morphogenetic Protein 2/therapeutic use-
dc.subject.MESHCollagen Type I/genetics-
dc.subject.MESHCollagen Type I/metabolism-
dc.subject.MESHCollagen Type II/genetics-
dc.subject.MESHCollagen Type II/metabolism-
dc.subject.MESHCytokines/pharmacology*-
dc.subject.MESHCytokines/therapeutic use*-
dc.subject.MESHFemale-
dc.subject.MESHFluorescent Antibody Technique-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-1/pharmacology-
dc.subject.MESHInterleukin-1/therapeutic use-
dc.subject.MESHIntervertebral Disc/drug effects*-
dc.subject.MESHIntervertebral Disc/pathology*-
dc.subject.MESHIntervertebral Disc Degeneration/drug therapy*-
dc.subject.MESHIntervertebral Disc Degeneration/genetics-
dc.subject.MESHIntervertebral Disc Degeneration/pathology*-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHOsteocalcin/genetics-
dc.subject.MESHOsteocalcin/metabolism-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHRecombinant Proteins/pharmacology-
dc.subject.MESHRecombinant Proteins/therapeutic use-
dc.subject.MESHSOX9 Transcription Factor/genetics-
dc.subject.MESHSOX9 Transcription Factor/metabolism-
dc.subject.MESHTransforming Growth Factor beta/pharmacology-
dc.subject.MESHTransforming Growth Factor beta/therapeutic use-
dc.subject.MESHTumor Necrosis Factor-alpha/pharmacology-
dc.titleBiologic response of degenerative living human nucleus pulposus cells to treatment with cytokines-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Neurosurgery (신경외과학)-
dc.contributor.googleauthorSang Hyun Kim-
dc.contributor.googleauthorSung Uk Kuh-
dc.contributor.googleauthorKeung Nyun Kim-
dc.contributor.googleauthorJeong Yoon Park-
dc.contributor.googleauthorKi Hong Cho-
dc.contributor.googleauthorDong Kyu Chin-
dc.contributor.googleauthorKeun Su Kim-
dc.contributor.googleauthorYong Eun Cho-
dc.identifier.doi10.3349/ymj.2015.56.1.277-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00196-
dc.contributor.localIdA00330-
dc.contributor.localIdA00331-
dc.contributor.localIdA01650-
dc.contributor.localIdA03979-
dc.contributor.localIdA03865-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid25510775-
dc.subject.keywordCytokine-
dc.subject.keywordIL-1β-
dc.subject.keywordTGF-β-
dc.subject.keywordTNF-α-
dc.subject.keyworddisc degeneration-
dc.subject.keywordrhBMP-2-
dc.contributor.alternativeNameKuh, Sung Uk-
dc.contributor.alternativeNameKim, Keun Su-
dc.contributor.alternativeNameKim, Keung Nyun-
dc.contributor.alternativeNamePark, Jeong Yoon-
dc.contributor.alternativeNameCho, Yong Eun-
dc.contributor.alternativeNameChin, Dong Kyu-
dc.contributor.affiliatedAuthorKuh, Sung Uk-
dc.contributor.affiliatedAuthorKim, Keun Su-
dc.contributor.affiliatedAuthorKim, Keung Nyun-
dc.contributor.affiliatedAuthorPark, Jeong Yoon-
dc.contributor.affiliatedAuthorChin, Dong Kyu-
dc.contributor.affiliatedAuthorCho, Yong Eun-
dc.rights.accessRightsfree-
dc.citation.volume56-
dc.citation.number1-
dc.citation.startPage277-
dc.citation.endPage286-
dc.identifier.bibliographicCitationYONSEI MEDICAL JOURNAL, Vol.56(1) : 277-286, 2015-
dc.identifier.rimsid56480-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers

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