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MicroRNA-30d and microRNA-181a regulate HOXA11 expression in the uterosacral ligaments and are overexpressed in pelvic organ prolapse

DC Field Value Language
dc.contributor.author김세광-
dc.contributor.author김호근-
dc.contributor.author문여정-
dc.contributor.author배상욱-
dc.contributor.author최종락-
dc.date.accessioned2016-02-04T11:00:12Z-
dc.date.available2016-02-04T11:00:12Z-
dc.date.issued2015-
dc.identifier.issn1582-1838-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/139451-
dc.description.abstractThe balanced turnover of collagen is necessary to maintain the mechanical strength of pelvic supportive connective tissues. Homeobox (HOX) A11 is a key transcriptional factor that controls collagen metabolism and homoeostasis in the uterosacral ligaments (USLs), and the deficient HOXA11 signalling may contribute to alterations in the biochemical strength of the USLs, leading to pelvic organ prolapse (POP). However, it is unknown how HOXA11 transcripts are regulated in the USLs. In this study, we found that microRNA (miRNA)-30d and 181a were overexpressed in women with POP, and their expression was inversely correlated with HOXA11 mRNA levels. The overexpression of miR-30d or 181a suppressed HOXA11 mRNA and protein levels in 293T cells, whereas the knockdown of these miRNAs enhanced HOXA11 levels and collagen production. Cotransfection of a luciferase reporter plasmid containing the 3'-untranslated region of HOXA11 with miR-30d or 181a mimic resulted in decreased relative luciferase activity. Conversely, cotransfection with anti-miR-30d or 181a increased luciferase activity. Taken together, these results indicate that both miR-30d and 181a are important posttranscriptional regulators of HOXA11 in the USLs and could be a potential therapeutic target for POP.-
dc.description.statementOfResponsibilityopen-
dc.format.extent501~509-
dc.relation.isPartOfJOURNAL OF CELLULAR AND MOLECULAR MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCell Line-
dc.subject.MESHConnective Tissue/metabolism-
dc.subject.MESHFemale-
dc.subject.MESHHEK293 Cells-
dc.subject.MESHHomeodomain Proteins/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHLigaments/metabolism*-
dc.subject.MESHMicroRNAs/metabolism*-
dc.subject.MESHMiddle Aged-
dc.subject.MESHPelvic Organ Prolapse/metabolism*-
dc.subject.MESHUterus/metabolism*-
dc.titleMicroRNA-30d and microRNA-181a regulate HOXA11 expression in the uterosacral ligaments and are overexpressed in pelvic organ prolapse-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학)-
dc.contributor.googleauthorMyung Jae Jeon-
dc.contributor.googleauthorEun Jae Kim-
dc.contributor.googleauthorMaria Lee-
dc.contributor.googleauthorHoguen Kim-
dc.contributor.googleauthorJong Rak Choi-
dc.contributor.googleauthorHee Dong Chae-
dc.contributor.googleauthorYeo Jung Moon-
dc.contributor.googleauthorSei Kwang Kim-
dc.contributor.googleauthorSang Wook Bai-
dc.identifier.doi10.1111/jcmm.12448-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00601-
dc.contributor.localIdA01183-
dc.contributor.localIdA01369-
dc.contributor.localIdA01793-
dc.contributor.localIdA04182-
dc.relation.journalcodeJ01302-
dc.identifier.eissn1582-4934-
dc.identifier.pmid25630974-
dc.subject.keywordHOXA11-
dc.subject.keywordmicroRNA-
dc.subject.keywordpelvic organ prolapse-
dc.subject.keyworduterosacral ligament-
dc.contributor.alternativeNameKim, Sei Kwang-
dc.contributor.alternativeNameKim, Ho Keun-
dc.contributor.alternativeNameMoon, Yeo Jung-
dc.contributor.alternativeNameBai, Sang Wook-
dc.contributor.alternativeNameChoi, Jong Rak-
dc.contributor.affiliatedAuthorKim, Sei Kwang-
dc.contributor.affiliatedAuthorKim, Ho Keun-
dc.contributor.affiliatedAuthorMoon, Yeo Jung-
dc.contributor.affiliatedAuthorBai, Sang Wook-
dc.contributor.affiliatedAuthorChoi, Jong Rak-
dc.rights.accessRightsfree-
dc.citation.volume19-
dc.citation.number2-
dc.citation.startPage501-
dc.citation.endPage509-
dc.identifier.bibliographicCitationJOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Vol.19(2) : 501-509, 2015-
dc.identifier.rimsid55403-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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