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ZBTB2 increases PDK4 expression by transcriptional repression of RelA/p65

Authors
 Min-Young Kim  ;  Dong-In Koh  ;  Won-Il Choi  ;  Bu-Nam Jeon  ;  Deok-yoon Jeong  ;  Kyung-Sup Kim  ;  Kunhong Kim  ;  Se-Hoon Kim  ;  Man-Wook Hur 
Citation
 NUCLEIC ACIDS RESEARCH, Vol.43(3) : 1609-1625, 2015 
Journal Title
 NUCLEIC ACIDS RESEARCH 
ISSN
 0305-1048 
Issue Date
2015
MeSH
Base Sequence ; Cell Line ; DNA Primers ; Humans ; Promoter Regions, Genetic ; Protein-Serine-Threonine Kinases/genetics* ; Real-Time Polymerase Chain Reaction ; Repressor Proteins/physiology* ; Sp1 Transcription Factor/metabolism ; Transcription Factor RelA/genetics* ; Transcription, Genetic*
Abstract
The NF-κB is found in almost all animal cell types and is involved in a myriad of cellular responses. Aberrant expression of NF-κB has been linked to cancer, inflammatory diseases and improper development. Little is known about transcriptional regulation of the NF-κB family member gene RelA/p65. Sp1 plays a key role in the expression of the RelA/p65 gene. ZBTB2 represses transcription of the gene by inhibiting Sp1 binding to a Sp1-binding GC-box in the RelA/p65 proximal promoter (bp, -31 to -21). Moreover, recent studies revealed that RelA/p65 directly binds to the peroxisome proliferator-activated receptor-γ coactivator1α (PGC1α) to decrease transcriptional activation of the PGC1α target gene PDK4, whose gene product inhibits pyruvate dehydrogenase (PDH), a key regulator of TCA cycle flux. Accordingly, we observed that RelA/p65 repression by ZBTB2 indirectly results in increased PDK4 expression, which inhibits PDH. Consequently, in cells with ectopic ZBTB2, the concentrations of pyruvate and lactate were higher than those in normal cells, indicating changes in glucose metabolism flux favoring glycolysis over the TCA cycle. Knockdown of ZBTB2 in mouse xenografts decreased tumor growth. ZBTB2 may increase cell proliferation by reprogramming glucose metabolic pathways to favor glycolysis by upregulating PDK4 expression via repression of RelA/p65 expression.
Files in This Item:
T201500374.pdf Download
DOI
10.1093/nar/gkv026
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Koh, Dong In(고동인)
Kim, Kun Hong(김건홍) ORCID logo https://orcid.org/0000-0001-5639-6372
Kim, Kyung Sup(김경섭) ORCID logo https://orcid.org/0000-0001-8483-8537
Kim, Min Young(김민영)
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Jeon, Bu Nam(전부남)
Choi, Won Il(최원일)
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139445
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