Cited 77 times in
PTPσ functions as a presynaptic receptor for the glypican-4/LRRTM4 complex and is essential for excitatory synaptic transmission
DC Field | Value | Language |
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dc.contributor.author | 엄지원 | - |
dc.date.accessioned | 2016-02-04T10:59:12Z | - |
dc.date.available | 2016-02-04T10:59:12Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139415 | - |
dc.description.abstract | Leukocyte common antigen-related receptor protein tyrosine phosphatases--comprising LAR, PTPδ, and PTPσ--are synaptic adhesion molecules that organize synapse development. Here, we identify glypican 4 (GPC-4) as a ligand for PTPσ. GPC-4 showed strong (nanomolar) affinity and heparan sulfate (HS)-dependent interaction with the Ig domains of PTPσ. PTPσ bound only to proteolytically cleaved GPC-4 and formed additional complex with leucine-rich repeat transmembrane protein 4 (LRRTM4) in rat brains. Moreover, single knockdown (KD) of PTPσ, but not LAR, in cultured neurons significantly reduced the synaptogenic activity of LRRTM4, a postsynaptic ligand of GPC-4, in heterologous synapse-formation assays. Finally, PTPσ KD dramatically decreased both the frequency and amplitude of excitatory synaptic transmission. This effect was reversed by wild-type PTPσ, but not by a HS-binding-defective PTPσ mutant. Our results collectively suggest that presynaptic PTPσ, together with GPC-4, acts in a HS-dependent manner to maintain excitatory synapse development and function. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1874~1879 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Analysis of Variance | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Brain/metabolism* | - |
dc.subject.MESH | Chromatography, Affinity | - |
dc.subject.MESH | Chromatography, High Pressure Liquid | - |
dc.subject.MESH | Gene Knockdown Techniques | - |
dc.subject.MESH | Genetic Vectors/genetics | - |
dc.subject.MESH | Glypicans/metabolism* | - |
dc.subject.MESH | Heparitin Sulfate/metabolism | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Immunoprecipitation | - |
dc.subject.MESH | In Situ Hybridization | - |
dc.subject.MESH | Mass Spectrometry | - |
dc.subject.MESH | Multiprotein Complexes/metabolism* | - |
dc.subject.MESH | Oligonucleotides/genetics | - |
dc.subject.MESH | Presynaptic Terminals/physiology | - |
dc.subject.MESH | Proteins/metabolism* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Receptor-Like Protein Tyrosine Phosphatases, Class 2/genetics | - |
dc.subject.MESH | Receptor-Like Protein Tyrosine Phosphatases, Class 2/metabolism* | - |
dc.subject.MESH | Synaptic Transmission/physiology* | - |
dc.title | PTPσ functions as a presynaptic receptor for the glypican-4/LRRTM4 complex and is essential for excitatory synaptic transmission | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Ji Seung Ko | - |
dc.contributor.googleauthor | Gopal Pramanik | - |
dc.contributor.googleauthor | Ji Won Um | - |
dc.contributor.googleauthor | Ji Seon Shim | - |
dc.contributor.googleauthor | Dongmin Lee | - |
dc.contributor.googleauthor | Kee Hun Kim | - |
dc.contributor.googleauthor | Gug-Young Chung | - |
dc.contributor.googleauthor | Giuseppe Condomitti | - |
dc.contributor.googleauthor | Ho Min Kim | - |
dc.contributor.googleauthor | Hyun Kim | - |
dc.contributor.googleauthor | Joris de Wit | - |
dc.contributor.googleauthor | Kang-Sik Park | - |
dc.contributor.googleauthor | Katsuhiko Tabuchi | - |
dc.contributor.googleauthor | Jaewon Ko | - |
dc.identifier.doi | 10.1073/pnas.1410138112 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02340 | - |
dc.relation.journalcode | J02550 | - |
dc.identifier.eissn | 1091-6490 | - |
dc.identifier.pmid | 25624497 | - |
dc.subject.keyword | LRRTM4 | - |
dc.subject.keyword | PTPσ | - |
dc.subject.keyword | glypican | - |
dc.subject.keyword | heparan sulfate | - |
dc.subject.keyword | synaptic cell adhesion | - |
dc.contributor.alternativeName | Um, Ji Won | - |
dc.contributor.affiliatedAuthor | Um, Ji Won | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 112 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1874 | - |
dc.citation.endPage | 1879 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.112(6) : 1874-1879, 2015 | - |
dc.identifier.rimsid | 55380 | - |
dc.type.rims | ART | - |
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