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A ratiometric fluorescent probe based on a coumarin-hemicyanine scaffold for sensitive and selective detection of endogenous peroxynitrite

Authors
 Xin Zhou  ;  Younghee Kwon  ;  Gyoungmi Kim  ;  Ji-Hwan Ryu  ;  Juyoung Yoon 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.64 : 285-291, 2015 
Journal Title
 BIOSENSORS & BIOELECTRONICS 
ISSN
 0956-5663 
Issue Date
2015
MeSH
Biosensing Techniques* ; Colorimetry ; Coumarins/chemistry* ; Fluorescent Dyes/chemistry* ; Peroxynitrous Acid/isolation & purification* ; Spectrometry, Fluorescence
Keywords
Cell imaging ; Fluorescent chemosensor ; ONOO(−) sensor ; Peroxynitrite sensor ; Reactive oxygen species probe
Abstract
In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO(-)) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO(-) that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO(-) with a low limit of detection LOD (49.7 nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO(-) in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO(-) in biology.
Full Text
http://www.sciencedirect.com/science/article/pii/S0956566314006952
DOI
10.1016/j.bios.2014.08.089
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers
Yonsei Authors
Kwon, Younghee(권영희)
Ryu, Ji Hwan(유지환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139165
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