Cited 52 times in
Washing-free heterogeneous immunosensor using proximity-dependent electron mediation between an enzyme label and an electrode
DC Field | Value | Language |
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dc.contributor.author | 김신영 | - |
dc.date.accessioned | 2015-12-28T11:16:57Z | - |
dc.date.available | 2015-12-28T11:16:57Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139103 | - |
dc.description.abstract | Washing processes, essential in most heterogeneous labeled assays, have been a big hurdle in simplifying the detection procedure and reducing assay time. Nevertheless, less attention has been paid to washing-free heterogeneous labeled assays. We report a purely washing-free immunosensor that allows fast, sensitive, and single-step detection of prostate-specific antigen in serum with low interference. Proximity-dependent electron mediation of ferrocenemethanol (Fc) between an indium-tin oxide (ITO) electrode and a glucose-oxidase (GOx) label allows us to discriminate between a bound and an unbound label: a bound label offers faster electron mediation than an unbound one. The electrooxidation of Fc at a low applied potential (0.13 V vs Ag/AgCl) and a low electrocatalytic ITO electrode and the oxidation of l-ascorbic acid by l-ascorbate oxidase minimize the effect of the interfering species. With a high concentration of glucose (200 mM), the signal and background levels are hardly dependent on the glucose-concentration variation in the sample. The washing-free immunosensor can detect a concentration of ca. 1 pg/mL for mouse IgG in phosphate-buffered saline and a concentration of ca. 10 pg/mL for prostate-specific antigen spiked in female serum after an incubation period of 10 min. The concentrations measured with actual clinical serum samples are in good agreement with the concentrations measured with a commercial instrument, which renders the washing-free heterogeneous immunosensor appealing for practical use. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Washing-free heterogeneous immunosensor using proximity-dependent electron mediation between an enzyme label and an electrode | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Gorachand Dutta | - |
dc.contributor.googleauthor | Sinyoung Kim | - |
dc.contributor.googleauthor | Seonhwa Park | - |
dc.contributor.googleauthor | Haesik Yang | - |
dc.identifier.doi | 10.1021/ac5006487 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00675 | - |
dc.relation.journalcode | J00136 | - |
dc.identifier.eissn | 1520-6882 | - |
dc.identifier.url | http://pubs.acs.org/doi/abs/10.1021/ac5006487 | - |
dc.contributor.alternativeName | Kim, Sin Young | - |
dc.contributor.affiliatedAuthor | Kim, Sin Young | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 86 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 4589 | - |
dc.citation.endPage | 4595 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, Vol.86(9) : 4589-4595, 2014 | - |
dc.identifier.rimsid | 52489 | - |
dc.type.rims | ART | - |
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