Cited 0 times in
Evaluation of the Selective Enrichment Culture to Recover Clostridium difficile
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김희정 | - |
dc.contributor.author | 이경원 | - |
dc.date.accessioned | 2015-12-28T11:15:05Z | - |
dc.date.available | 2015-12-28T11:15:05Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1738-3544 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139031 | - |
dc.description.abstract | To evaluate the recovery rates to increase toxigenic C. difficile, the selective enrichment broth culture methods were compared with commonly used cytotoxin assays and toxigenic culture. First, the enrichment culture, using the selective medium broth for 2 to 5 days, was performed and then, toxigenic C. difficile was confirmed by C. difficile toxin gene-specific PCR after being cultured on C. difficile selective agar. The sensitivity of C. difficile from the enrichment culture (100%) was higher than that of C. difficile selective agar culture (93.8%), while positive predictive values (PPV) were low; 72.7% (16/22) and 88.2% (15/17). PPV of the enrichment culture are not high. Recently, combinations of C. difficile selective agar culture, C. difficile A & B assays, glutamate dehydrogenase, and nucleic acid amplification method are widely used. The enrichment culture was disadvantageous in PPV, turn-around time, and cost. So, what we performed is not considered as a common method of diagnosis of C. difficile-associated diarrhea. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 140~142 | - |
dc.relation.isPartOf | Korean Journal of Clinical Laboratory Science | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Evaluation of the Selective Enrichment Culture to Recover Clostridium difficile | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Byoungrak An | - |
dc.contributor.googleauthor | Heejung Kim | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.identifier.doi | 10.15324/kjcls.2014.46.4.140 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A01219 | - |
dc.relation.journalcode | J01982 | - |
dc.subject.keyword | C. difficile | - |
dc.subject.keyword | Enrichment culture | - |
dc.subject.keyword | Toxigenic culture | - |
dc.contributor.alternativeName | Kim, Hee Jung | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Kim, Heejung | - |
dc.citation.volume | 46 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 140 | - |
dc.citation.endPage | 142 | - |
dc.identifier.bibliographicCitation | Korean Journal of Clinical Laboratory Science, Vol.46(4) : 140-142, 2014 | - |
dc.identifier.rimsid | 52435 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.