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Cell-cycle-regulated activation of Akt kinase by phosphorylation at its carboxyl terminus.

Authors
 Pengda Liu  ;  Michael Begley  ;  Wojciech Michowski  ;  Hiroyuki Inuzuka  ;  Miriam Ginzberg  ;  Daming Gao  ;  Peiling Tsou  ;  Wenjian Gan  ;  Antonella Papa  ;  Byeong Mo Kim  ;  Lixin Wan  ;  Amrik Singh  ;  Bo Zhai  ;  Min Yuan  ;  Zhiwei Wang  ;  Steven P. Gygi  ;  Tae Ho Lee  ;  Kun Ping Lu  ;  Alex Toker  ;  Pier Paolo Pandolfi  ;  John M. Asara  ;  Marc W. Kirschner  ;  Piotr Sicinski  ;  Lewis Cantley  ;  Wenyi Wei 
Citation
 NATURE, Vol.508(7497) : 541-545, 2014 
Journal Title
 NATURE 
ISSN
 0028-0836 
Issue Date
2014
MeSH
Animals ; Apoptosis/genetics ; Cell Cycle/physiology* ; Cell Proliferation ; Cyclin A2/metabolism ; Cyclin-Dependent Kinase 2/metabolism ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Enzyme Activation ; Male ; Mechanistic Target of Rapamycin Complex 2 ; Mice ; Multiprotein Complexes/metabolism ; Neoplasms/enzymology ; Neoplasms/pathology ; Olfactory Bulb/cytology ; Olfactory Bulb/enzymology ; Olfactory Bulb/metabolism ; Oncogene Protein v-akt/chemistry ; Oncogene Protein v-akt/metabolism ; Phosphorylation ; Phosphoserine/metabolism ; Phosphothreonine/metabolism ; Proto-Oncogene Proteins c-akt/chemistry* ; Proto-Oncogene Proteins c-akt/metabolism* ; TOR Serine-Threonine Kinases/metabolism
Abstract
Akt, also known as protein kinase B, plays key roles in cell proliferation, survival and metabolism. Akt hyperactivation contributes to many pathophysiological conditions, including human cancers, and is closely associated with poor prognosis and chemo- or radiotherapeutic resistance. Phosphorylation of Akt at S473 (ref. 5) and T308 (ref. 6) activates Akt. However, it remains unclear whether further mechanisms account for full Akt activation, and whether Akt hyperactivation is linked to misregulated cell cycle progression, another cancer hallmark. Here we report that Akt activity fluctuates across the cell cycle, mirroring cyclin A expression. Mechanistically, phosphorylation of S477 and T479 at the Akt extreme carboxy terminus by cyclin-dependent kinase 2 (Cdk2)/cyclin A or mTORC2, under distinct physiological conditions, promotes Akt activation through facilitating, or functionally compensating for, S473 phosphorylation. Furthermore, deletion of the cyclin A2 allele in the mouse olfactory bulb leads to reduced S477/T479 phosphorylation and elevated cellular apoptosis. Notably, cyclin A2-deletion-induced cellular apoptosis in mouse embryonic stem cells is partly rescued by S477D/T479E-Akt1, supporting a physiological role for cyclin A2 in governing Akt activation. Together, the results of our study show Akt S477/T479 phosphorylation to be an essential layer of the Akt activation mechanism to regulate its physiological functions, thereby providing a new mechanistic link between aberrant cell cycle progression and Akt hyperactivation in cancer.
Files in This Item:
T201405576.pdf Download
DOI
10.1038/nature13079
Appears in Collections:
5. Research Institutes (연구소) > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단) > 1. Journal Papers
Yonsei Authors
Kim, Byeong Mo(김병모) ORCID logo https://orcid.org/0000-0002-0582-3132
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138762
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