Cited 11 times in
Silencing of hypoxia-inducible factor-1β induces anti-tumor effects in hepatoma cell lines under tumor hypoxia.
DC Field | Value | Language |
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dc.contributor.author | 박준용 | - |
dc.contributor.author | 안상훈 | - |
dc.contributor.author | 이미솔 | - |
dc.contributor.author | 한광협 | - |
dc.contributor.author | 강원석 | - |
dc.contributor.author | 김도영 | - |
dc.contributor.author | 김승업 | - |
dc.contributor.author | 김승택 | - |
dc.date.accessioned | 2015-12-28T11:06:26Z | - |
dc.date.available | 2015-12-28T11:06:26Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/138706 | - |
dc.description.abstract | Dimerization of hypoxia-inducible factor-1 beta (HIF-1β) [aryl hydrocarbon receptor nuclear translocator (ARNT)] with HIF-1α is involved in various aspects of cancer biology, including proliferation and survival under hypoxic conditions. We investigated the in vitro mechanism by which silencing of HIF-1β leads to the suppression of tumor cell growth and cellular functions. Various hepatocellular carcinoma (HCC) cell lines (Huh-7, Hep3B, and HepG2) were transfected with small interfering RNA (siRNA) against HIF-1β (siHIF-1β) and cultured under hypoxic conditions (1% O2 for 24 h). The expression levels of HIF-1β, HIF-1α, and growth factors were examined by immunoblotting. Tumor growth was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and tumor activity was measured by terminal deoxynucleotidyl transferase dUTP nick end labeling, tumor cell invasion, and migration assays. Under hypoxic conditions, silencing of HIF-1β expression suppressed tumor cell growth and regulated the expression of tumor growth-related factors, such as vascular endothelial growth factor, epidermal growth factor, and hepatocyte growth factor. Suppression of tumor cell invasion and migration was also demonstrated in HIF-1β-silenced HCC cell lines. Silencing of HIF-1β expression may induce anti-tumor effects under hypoxic conditions in HCC cell lines. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | e103304 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis/genetics | - |
dc.subject.MESH | Aryl Hydrocarbon Receptor Nuclear Translocator/genetics* | - |
dc.subject.MESH | Aryl Hydrocarbon Receptor Nuclear Translocator/metabolism | - |
dc.subject.MESH | Carcinoma, Hepatocellular/genetics | - |
dc.subject.MESH | Carcinoma, Hepatocellular/metabolism | - |
dc.subject.MESH | Carcinoma, Hepatocellular/pathology | - |
dc.subject.MESH | Cell Hypoxia | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Movement/genetics | - |
dc.subject.MESH | Cell Proliferation/genetics* | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic* | - |
dc.subject.MESH | Hep G2 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypoxia-Inducible Factor 1, alpha Subunit/genetics | - |
dc.subject.MESH | Hypoxia-Inducible Factor 1, alpha Subunit/metabolism | - |
dc.subject.MESH | Immunoblotting | - |
dc.subject.MESH | Liver Neoplasms/genetics | - |
dc.subject.MESH | Liver Neoplasms/metabolism | - |
dc.subject.MESH | Liver Neoplasms/pathology | - |
dc.subject.MESH | RNA Interference* | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.title | Silencing of hypoxia-inducible factor-1β induces anti-tumor effects in hepatoma cell lines under tumor hypoxia. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Sung Hoon Choi | - |
dc.contributor.googleauthor | Ae Ri Chung | - |
dc.contributor.googleauthor | Wonseok Kang | - |
dc.contributor.googleauthor | Jun Yong Park | - |
dc.contributor.googleauthor | Mi Sol Lee | - |
dc.contributor.googleauthor | Shin Won Hwang | - |
dc.contributor.googleauthor | Do Young Kim | - |
dc.contributor.googleauthor | Seung Up Kim | - |
dc.contributor.googleauthor | Sang Hoon Ahn | - |
dc.contributor.googleauthor | Seungtaek Kim | - |
dc.contributor.googleauthor | Kwang Hyub Han | - |
dc.identifier.doi | 10.1371/journal.pone.0103304 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01675 | - |
dc.contributor.localId | A02226 | - |
dc.contributor.localId | A02771 | - |
dc.contributor.localId | A04268 | - |
dc.contributor.localId | A00061 | - |
dc.contributor.localId | A00654 | - |
dc.contributor.localId | A00661 | - |
dc.contributor.localId | A00385 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 25068796 | - |
dc.contributor.alternativeName | Park, Jun Yong | - |
dc.contributor.alternativeName | Ahn, Sang Hoon | - |
dc.contributor.alternativeName | Lee, Mi Sol | - |
dc.contributor.alternativeName | Han, Kwang Hyup | - |
dc.contributor.alternativeName | Kang, Won Suk | - |
dc.contributor.alternativeName | Kim, Do Young | - |
dc.contributor.alternativeName | Kim, Seung Up | - |
dc.contributor.alternativeName | Kim, Seung Taek | - |
dc.contributor.affiliatedAuthor | Park, Jun Yong | - |
dc.contributor.affiliatedAuthor | Ahn, Sang Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Mi Sol | - |
dc.contributor.affiliatedAuthor | Han, Kwang Hyup | - |
dc.contributor.affiliatedAuthor | Kang, Won Suk | - |
dc.contributor.affiliatedAuthor | Kim, Seung Up | - |
dc.contributor.affiliatedAuthor | Kim, Seung Taek | - |
dc.contributor.affiliatedAuthor | Kim, Do Young | - |
dc.citation.volume | 9 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | e103304 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.9(7) : e103304, 2014 | - |
dc.identifier.rimsid | 39374 | - |
dc.type.rims | ART | - |
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