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Highly fluorescent conjugated polyelectrolyte for protein sensing and cell-compatible chemosensing applications

Authors
 Jungmok You  ;  Teahoon Park  ;  Jeonghun Kim  ;  June Seok Heo  ;  Han-Soo Kim  ;  Hyun Ok Kim  ;  Eunkyoung Kim 
Citation
 ACS Applied Materials & Interfaces, Vol.6(5) : 3305-3311, 2014 
Journal Title
 ACS Applied Materials & Interfaces 
ISSN
 1944-8244 
Issue Date
2014
Abstract
Using a highly fluorescent, water-soluble polymer derived from a triazine-bridged copolymer (DTMSPV), we explored the tunable fluorescence properties of the water-soluble DTMSPV by solvent polarity to function as a fluorescence sensory probe for protein sensing. The green-blue fluorescence from DTMSPV was significantly enhanced in the presence of bovine serum albumin through hydrophobic interactions. Meanwhile, complete quenching of the fluorescence from DTMSPV occurred in the presence of hemoglobin through iron complexation with the polyelectrolyte. In addition, the DTMSPVs were highly fluorescent and permeated into living mesenchymal stem cells (MSCs), enabling effective imaging of the MSCs. This permeation into stem cells is crucial to the detection of Al(3+) in living MSCs. The interaction between the triazine units in DTMSPV with the Al(3+) ions allows for the detection of Al(3+) in living cells. Thus, a strong fluorescence from living MSCs pretreated with DTMSPV was quenched as a function of the Al(3+) concentration, confirming that DTMSPV is a cell-permeable fluorescent polymer that can function as a versatile probe to detect Al(3+) in living cells.
Full Text
http://pubs.acs.org/doi/abs/10.1021/am500269t
DOI
10.1021/am500269t
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실)
Yonsei Authors
김한수(Kim, Han Soo)
김현옥(Kim, Hyun Ok) ORCID logo https://orcid.org/0000-0002-4964-1963
허준석(Heo, June Seok)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/138671
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