흉선이 난소 '아렐기-'성변화에 미치는 영향에 관한 실험적 연구

Abstract

[한글]

EXPERIMENTAL STUDIES ON THE INFLUENCE OF THE THYMUS GLAND ON ALLERGIC CHANGES IN

OVARY

TAI SIK HWANGM M. D.

From the Department of Obsterics and Gynecology,

School of Medicine, The University of Yunsei

There have been many reports on the inter-relationship between the thymus and

other endocrine organs. These reports seem to suggest a close tie between the

functions of the thymus and the growth and function of the ovaries.

Conflicting opinions have been expressed by different workers in regard to the

influence of the thymus on allergic phenomena. Some maintain that the thymus plays

a part in acceleration the allergic phenomena and others state that the thymus

depresses such activities.

The author has investigated the influence of the thymus on allergic phenomena of

the ovaries. Present report deals with the outcome of this investigative works

Material :

Young, female rabbits, under 1 kg. of body weight, were used in this experiment.

Fresh horse serum prepared by National Vaccine Laboratory were used as the antigen

for sensitization.

Method :

The rabbits were divided into four groups as follows :

Group 1 : Normal Control

Group 2 : Thymectomized animals

Group 3 : Animals thymectomized and the thymus autotransplanted.

Group 4 : Animals with homotransplantation of thymus

Each group was subdivided into animals that had been sensitized with horse serum

and one that had not been sensitized.

Sensitization :

The animals were sensitized by injection of 1.0cc horse serum per kg. body weight

subcutaneously for 4 days. The site of injection was the back. Fifteen days

following the last injection the animal was injected with 0.1cc horse serum per kg.

weight into one of the ovaries.

The non-sensitized group received only 0.1 cc per kg. body weight of the antigen

into one of the ovaries.

Animals from all groups, the sensitized and non-sensitized, were then sacrificed

at the end of 3 hours, 6 hours, 12 hours, 24 hours, 72 hours, 1 week, and 2 weeks.

The section were prepared by Hematoxylin-Eosin. Van Gieson, and Sudam Ⅲ Stains

and were submitted for histological examination.

Results :

Following changes were observed :

1) Normal Control

The allergic changes were observed at the end of 12 hours following the

reinjection.

These were : a) dilatation and congestion of blood vessels. b) hydropic swelling

of the perivascular connective tissues. c) perivascular infiltration of

pseudoeosinophilic leucocytes and small round cells. d) perivascular retention of

inflammatory exudates. e) stasis and thrombosis within the vessels. f) separation

of the endothelial cells lining the vessels. g) fibrinoid degeneration of the

vessel walls. h) Theca cell layer and granulosa cell layer of the follicles

revealed cloudy swelling, necrobiosis, pseudo-eosinophilic leucocytes and small

round cell infiltration.

And at the end of 24 hours, these changes were most pronounced. After 48 hours,

these changes begin to subside in intensity with decrease in the number of

pseudo-eosinophilic leucocytes and small round cells. At the same time, there

appeared histiocytes in the picture and proliferation of perivascular connective

tissues. At the end of two weeks following the reinjection, only remaining changes

were slight dilatation of blood vessels, proliferation of interstitial connective

tissues and fibrosis.

2) Thymectomized Animals

The allergic changes in the ovary from these animals were much less in intensity

than those of the normal control group.

3) Animals thymectomized and the thymus autotransplantation.

The same changes were found in this group as in the control, but the degree of

changes was less pronounced.

4) Animals with homotransplantation of thymus

The same changes were observed in this group as in the control but the changes

were most pronounced.

Conclusion

Thymectomy has an inhibitory effect on the allergic changes of the ovary and

homotransplantation of thymus accelerates these allergic changes.

[영문]

There have been many reports on the inter-relationship between the thymus and other endocrine organs. These reports seem to suggest a close tie between the functions of the thymus and the growth and function of the ovaries.

Conflicting opinions have been expressed by different workers in regard to the influence of the thymus on allergic phenomena. Some maintain that the thymus plays a part in acceleration the allergic phenomena and others state that the thymus depresses such activities.

The author has investigated the influence of the thymus on allergic phenomena of the ovaries. Present report deals with the outcome of this investigative works Material :

Young, female rabbits, under 1 kg. of body weight, were used in this experiment.

Fresh horse serum prepared by National Vaccine Laboratory were used as the antigen for sensitization.

Method :

The rabbits were divided into four groups as follows :

Group 1 : Normal Control

Group 2 : Thymectomized animals

Group 3 : Animals thymectomized and the thymus autotransplanted.

Group 4 : Animals with homotransplantation of thymus

Each group was subdivided into animals that had been sensitized with horse serum and one that had not been sensitized.

Sensitization :

The animals were sensitized by injection of 1.0cc horse serum per kg. body weight subcutaneously for 4 days. The site of injection was the back. Fifteen days following the last injection the animal was injected with 0.1cc horse serum per kg. weight into one of the ovaries.

The non-sensitized group received only 0.1 cc per kg. body weight of the antigen into one of the ovaries.

Animals from all groups, the sensitized and non-sensitized, were then sacrificed at the end of 3 hours, 6 hours, 12 hours, 24 hours, 72 hours, 1 week, and 2 weeks.

The section were prepared by Hematoxylin-Eosin. Van Gieson, and Sudam Ⅲ Stains and were submitted for histological examination.

Results :

Following changes were observed :

1) Normal Control

The allergic changes were observed at the end of 12 hours following the reinjection.

These were : a) dilatation and congestion of blood vessels. b) hydropic swelling of the perivascular connective tissues. c) perivascular infiltration of pseudoeosinophilic leucocytes and small round cells. d) perivascular retention of inflammatory exudates. e) stasis and thrombosis within the vessels. f) separation of the endothelial cells lining the vessels. g) fibrinoid degeneration of the vessel walls. h) Theca cell layer and granulosa cell layer of the follicles revealed cloudy swelling, necrobiosis, pseudo-eosinophilic leucocytes and small

round cell infiltration.

And at the end of 24 hours, these changes were most pronounced. After 48 hours, these changes begin to subside in intensity with decrease in the number of pseudo-eosinophilic leucocytes and small round cells. At the same time, there appeared histiocytes in the picture and proliferation of perivascular connective

tissues. At the end of two weeks following the reinjection, only remaining changes were slight dilatation of blood vessels, proliferation of interstitial connective tissues and fibrosis.

2) Thymectomized Animals

The allergic changes in the ovary from these animals were much less in intensity than those of the normal control group.

3) Animals thymectomized and the thymus autotransplantation.

The same changes were found in this group as in the control, but the degree of changes was less pronounced.

4) Animals with homotransplantation of thymus

The same changes were observed in this group as in the control but the changes were most pronounced.

Conclusion

Thymectomy has an inhibitory effect on the allergic changes of the ovary and homotransplantation of thymus accelerates these allergic changes.