Effects of alginate gel-encapsulated adenoviruses expressing relaxin on scar remodeling in a pig model
Other Titles
Alginate gel에 포획된 Relaxin을 분비하는 아데노바이러스가 돼지의 반흔 성숙 과정에 미치는 영향
Authors
윤인식
Department
Dept. of Plastic and Reconstructive Surgery (성형외과학교실)
Issue Date
2014
Description
Dept. of Medicine/박사
Abstract
To evaluate the effects of relaxin on scar remodeling, we investigated the scar remodeling process after injecting alginate gel-encapsulated adenoviruses expressing relaxin using a pig scar model.In this study, we divided the scars into three groups and the injection was done according to the groups, respectively. Group I: alginate gel alone, group II: alginate gel + adenoviruses not containing the relaxin gene (dE1-RGD/LacZ), group III: alginate gel + adenoviruses expressing the relaxin gene (dE1-RGD/LacZ/RLX). The surface areas, color and pliability of scars were compared, and various factors influencing scar formation, collagen arrangement, and mast cell counts were quantitatively analyzed using RT-PCR and immunohistochemical staining.The surface areas of scars decreased, color was improved, pliability increased, mast cell number decreased, and a more mature collagen arrangement was observed in the group III. The expression levels of transforming growth factor (TGF)-β1, tissue inhibitor of metalloproteinase (TIMP)1, and α-smooth muscle actin (SMA) were reduced in the tissues of the group III, whereas the expression levels of TGF-β3 and matrix metalloproteinase (MMP)1 increased. Additionally, the expression of major extracellular matrix (ECM) components was decreased.Alginate gel-encapsulated adenoviruses expressing relaxin improved the surface area, color, and pliability of scars. Myofibroblasts were likely inhibited by relaxin, which either reduces the expression of TGF-β1 and TIMP1, or increases the expression of TGF-β3 and MMP1; scar remodeling was also enhanced as mast cell number decreased.