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Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury
DC Field | Value | Language |
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dc.contributor.author | 임재열 | - |
dc.date.accessioned | 2015-12-24T09:42:14Z | - |
dc.date.available | 2015-12-24T09:42:14Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/136363 | - |
dc.description | Dept. of Medicine/박사 | - |
dc.description.abstract | Vocal fold (VF) scarring remains a therapeutic challenge. Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates epithelial wound healing, and recently, growth factor therapy has been applied to promote tissue repair. This study was undertaken to investigate the effect of GM-CSF on VF wound healing in vivo and in vitro. VF scarring was induced in New Zealand white rabbits by direct injury. Either GM-CSF (treatment group) or PBS (control group) was injected into the VFs of rabbits immediately post-injury. Endoscopic, histopathological, immunohistochemical, and biomechanical evaluations of VFs were performed at 3 months post-injury. Human vocal fold fibroblasts (hVFFs) were cultured with GM-CSF. Productions of collagens type I and III were examined immunocytochemically and the syntheses of elastin and hyaluronic acids were evaluated by ELISA. The mRNA levels of genes related to ECM components and ECM production-related growth factors, such as HGF and TGF-ß1 were examined by real time RT-PCR.The GM-CSF-treated VFs showed reduced collagen deposition compared with the PBS-injected controls. Immunohistochemical staining revealed the amount of type I collagen and fibronectin was less in the GM-CSF-treated VFs (P = 0.0449 and P = 0.018, respectively). Viscous and elastic shear moduli of VF samples were significantly lower in the GM-CSF group than in the PBS-injected group (P < 0.0001 and P < 0.01, respectively). Mucosal waves in GM-CSF group showed significant improvement compared with the PBS group (P = 0.0446). GM-CSF inhibited TGF-β1-induced collagen synthesis by hVFFS (P < 0.05) and the production of hyaluronic acids increased at 72 hours post-treatment. The expressions of the mRNAs of HAS-2, tropoelastin, MMP-1, HGF, and c-Met were significantly increased by GM-CSF though at different time points (P < 0.05). The study shows that GM-CSF has therapeutic potential for the remodeling of VF wound and the promotion of VF regeneration. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Regulation of wound healing by granulocyte-macrophage colony-stimulating factor after vocal fold injury | - |
dc.title.alternative | GM-CSF의 성대 손상 후 성대 창상치유 촉진 조절을 통한 성대반흔 치료 | - |
dc.type | Thesis | - |
dc.identifier.url | https://ymlib.yonsei.ac.kr/catalog/search/book-detail/?cid=CAT000000129940 | - |
dc.contributor.alternativeName | Lim, Jea Yol | - |
dc.type.local | Dissertation | - |
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