탈회된 자가골과 동종골 및 이종골 이식에 관한 실험적 연구

Abstract

[영문]

[한글]

탈회골의 골형성 유도능력을 자가골과 동종골 및 이종골에서 서로 비교하기 위하여 동

종골로서는 백서, 이종골로서는 가토의 탈회골 분말 및 골편을 이식한 후 4일째부터 5일

간격으로 34일까지의 골화과정에서 시기별 조직학적 소견의 차이를 광학현미경으로 관찰

하고 연골 및 신생골 형성능력을 정량적으로 비교해 본 결과 다음과 같은 결론을 얻었다.

1. 탈회된 자가골과 동종골 및 이종골의 골편과 분말을 이식한 후 나타나는 골형성 과

정은 모두 연골내골화였다.

2. 탈회된 골분말을 이식한 경우 골형성 과정은 자가골과 동종골에서 시기적으로나 양

적으로 뚜렷한 차이가 없었으며, 이종골에서는 동종골보다 시기적으로 지연되었고 적은

양의 골이 형성되었다.

3. 탈회된 골편을 이식한 경우 신생골의 양은 자가골이나 동종골에서 이종골보다 뚜렷

하게 증가되었고 이종골에서는 시기적으로 지연되는 소견을 보였으며, 골형성 과정은 골

분말을 이식한 경우보다 시기적으로 지연되었고 신생골의 양도 매우 적었다.

4. 탈회된 골편을 이식한 경우 연골 및 신생골 형성은 골측부보다 골단부에서 활발하게

나타났다.

A comparative Study of Induced Osteogenesis by Implantation of Decalcified

Autogenous, Homogenous, Heterogenous Bone

In Chang Cho

Department of Medical Science The Graduate School, Yonsei University

(Directed by Prof. Tai Sun Shin M.D. and Prof. Young Ho Lee, M.D.)

Various methods of bone graft have been suggested for dealing with cavities or

losses of substance in bones, whether caused by disease, accident or operation.

Fresh living autograft has the most favorable effect, but it has much problems in

limitation of amount and various morbidities. Allograft or xenograft of bone has

significant rates of complication. As the method of reducing antigenicity,

decalcified bone grafts ahs been reported with good results in experimental

animals. Decalcified-devitalized bone grafts provide a scaffoling, but contain no

living cells. Under a certain set of condition, decalcified bone graft changes the

phenotype of fibroblasts adjacent to the implant and the responding fibroblast

emerge as chondroblasts or osteoblasts and their fate is altered permernantly.

But the transforming factor is not well identified yet.

This paper presents a series of experiments employing decalcification technique

to compare autogenic, allogenic and xenogenic transplantation.

Induced osteogenesis by the decalcified bone implantation was compared in

autograft allograft and xenograft.

As experimental method, decalcified bone powders and bone chips(0.5x1x3 mm sized)

was miplanted in the subcutaneous tissue of rat's abdomen.

Histological observation and comparative analysis was carried out to find out the

differences in processes of induced osteogenesis and quantity of new bone

periodically.

The results were as follows:

1. Osteogenesis induced by the decalcified bone graft was endochondral

ossification type.

2. Cartilage and new bone formation was also delayed and less amount in xenograft

than autograft and allograft.

3. New bone formation was delayed in bone chip than bone powder and new bone

formation was too small to be compared.

4. In the bone chip implantation, cartilage and new bone formation was more

active in the end portion of decalcified bone than the side.