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Serum dickkopf-1 (DKK-1) as a biomarker for the diagnosis of hepatocellular carcinoma
DC Field | Value | Language |
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dc.contributor.author | 김승업 | - |
dc.date.accessioned | 2015-12-24T09:05:51Z | - |
dc.date.available | 2015-12-24T09:05:51Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/134949 | - |
dc.description | Dept. of Medicine/박사 | - |
dc.description.abstract | Backgrounds & Aims: Dickkopf-1 (DKK-1) is a Wnt/β-catenin signaling pathway inhibitor. We investigated whether DKK-1 is related to progression in hepatocellular carcinoma (HCC) cells and HCC patients. Methods: In vitro reverse-transcription (RT)-PCR, wound healing assays, and invasion assays, and ELISAs of patient serum samples were employed. The diagnostic accuracy of the serum DKK-1 ELISA was assessed using receiver operating characteristic (ROC) curves and area under ROC (AUC) analyses.Results: RT-PCR showed high DKK-1 expression in Hep3B and low in 293 cells. Similarly, the secreted DKK-1 concentration in the culture media was high in Hep3B and low for 293 cells. Wound healing and invasion assay using 293, Huh7, and Hep3B cells showed that DKK-1 overexpression promoted cell migration and invasion, whereas DKK-1 knock-down inhibited them. When serum DKK-1 level was assessed in 370 participants (217 with HCC and 153 without), it was significantly higher in HCC patients than in control groups (median 1.48 vs. 0.90 ng/mL, P<0.001). The optimum DKK-1 cutoff level was 1.01 ng/mL (AUC=0.829; sensitivity 90.7%; specificity 62.0%). DKK-1 had a greater AUC in diagnosing HCC than alpha-fetoprotein (AFP) and des-gamma-carboxy prothrombin (DCP) (AUC=0.829 vs. 0.794 and 0.815, respectively). When all three biomarkers were combined (DKK-1+AFP+DCP), they showed remarkably increased accuracy (AUC=0.952). Conclusion: DKK-1 might be a key regulator in HCC progression and a potential therapeutic target in HCC. In addition, serum DKK-1 could complement the diagnostic accuracy of AFP and DCP. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Serum dickkopf-1 (DKK-1) as a biomarker for the diagnosis of hepatocellular carcinoma | - |
dc.title.alternative | 혈청 dickkopf-1 (DKK-1)의 간세포암 진단을 위한 바이오마커로서의 유용성 | - |
dc.type | Thesis | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.localId | A00654 | - |
dc.contributor.alternativeName | Kim, Seung Up | - |
dc.contributor.affiliatedAuthor | 김승업 | - |
dc.type.local | Dissertation | - |
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