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MicroRNA-133a inhibits norepinephrine-induced cardiac hypertrophy via suppression of PKC signaling
DC Field | Value | Language |
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dc.contributor.author | 문재연 | - |
dc.date.accessioned | 2015-12-24T08:47:43Z | - |
dc.date.available | 2015-12-24T08:47:43Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/134240 | - |
dc.description | Dept. of Medicine/박사 | - |
dc.description.abstract | Cardiac hypertrophy is associated with the development of heart failure and has been known as a predictor for cardiovascular morbidity and mortality. A recent study unveiled potential regulatory roles for microRNA-133a (miR-133a) in cardiac hypertrophy. However, it has not been studied to elucidate the connection between miR-133a and norepinephrine-induced cardiomyocyte hypertrophy. Here, we investigated the expression and functional role of miR-133a in a norepinephrine-induced hypertrophic cardiomyocyte and determined the target of miR-133a in hypertrophic signaling. Neonatal rat cardiomyocytes were isolated, and cardiac hypertrophy was induced by treatment with 10 μM norepinephrine. In this study, we determined that miR-133a plays a pivotal role in the regulation of norepinephrine-induced cardiac hypertrophy. miR-133a expression is inversely related to cardiac hypertrophy, and the treatment with miR-133a mimics before norepinephrine management prevents norepinephrine-induced cardiac hypertrophy. Second, we found that the direct target of miR-133a is Protein Kinase C δ (PKCδ), which was confirmed by both Luciferase assay and Western blot analysis. We found no relationship between miR-133a and G protein. Finally, after transfection with miR-133a, MEK and ERK, which are in the downstream pathway, and proto-oncogenes such as c-fos, c-myc and c-jun were down-regulated in hypertrophic cardiomyocytes, suggesting that PKCδ is a novel target of miR-133a in cardiomyocytes, and that administration of or treatment with miR-133a mimics could be used in the future as a therapeutic application in the clinical setting | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | MicroRNA-133a inhibits norepinephrine-induced cardiac hypertrophy via suppression of PKC signaling | - |
dc.title.alternative | miR-133a에 의한 노에피네프린으로 유도된 심근비대 억제효과 | - |
dc.type | Thesis | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.localId | A01376 | - |
dc.contributor.alternativeName | Moon, Jae Youn | - |
dc.contributor.affiliatedAuthor | 문재연 | - |
dc.type.local | Dissertation | - |
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