Proteomic profiling of T cells from patients with intrinsic atopic dermatitis and extrinsic atopic dermatitis

Abstract

Background Atopic dermatitis (AD) is a highly pruritic, chronic relapsing inflammatory skin disease. The pathogenesis of AD has been known to be altered skin barrier and immune dysregulation. As new subsets of T cells are recently introduced as contributing factors to the immune dysregulation of AD, variable subsets of T cells play pivotal roles in the pathogenesis of AD. Objective To understand the role of T cells in the pathogenesis of AD between different AD subsets, differentialy expressed proteins (DEPs) of T cells were compared from patients with extrinsic AD, intrinsic AD and healthy controls (HC) Methods For proteomic analysis, normalized spectral index quantification and tandem mass tag (TMT) labeling based quantification were used. For following validation western blotting and fluorescence activated cell sorting was used. Results We identified several DEPs between extrinsic, intrinsic AD and HCs. Among them, the upregulation of galectin-10 and S100A9 were validated by western blotting analysis. In addition, galectin-10 expressing CD3+T cells showed higher expression of IL-22 in AD than in HC. Conclusion Based on proteomic analysis comparing the extrinsic, intrinsic AD and HCs, several DEPs that might be related with pathogenesis were identified. By following validation study, galectin-10 was shown to have a role in the pathogenesis of AD in association with Th22.