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Characterization of signaling pathways for immune response induced by a recombinant lipoprotein, IlpA of Vibrio vulnificus
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dc.contributor.author | 이나연 | - |
dc.date.accessioned | 2015-12-24T08:42:43Z | - |
dc.date.available | 2015-12-24T08:42:43Z | - |
dc.date.issued | 2011 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/134048 | - |
dc.description | Dept. of Medical Science/박사 | - |
dc.description.abstract | Vibrio vulnificus is a pathogenic bacterium that causes primary septicemia, which follows a classical septic shock pathway including an overwhelming inflammatory cytokine response. V. vulnificus IlpA is a potent immunogenic lipoprotein that triggers cytokine production in human monocytes by activating the toll-like receptor 2 (TLR2). In this study, we further defined the IlpA signaling pathways involved in cytokine production in the human monocytic cell line, THP-1. TLR2 was involved in cytokine production by complexing with TLR1, but not TLR6. MyD88 was necessary for IlpA-induced cytokine expression through TLR1/2. Three mitogen activated protein kinases (MAPKs), p38, ERK1/2, and JNK, were activated in THP-1 cells stimulated with recombinant IlpA (rIlpA). Selective inhibition of each MAPK resulted in significant decrease of rIlpA-induced cytokine production. Especially, functional TLR2 was necessary for IlpA-induced activation of p38 and JNK. IlpA augmented the DNA-binding activity of nuclear factor-kappaB (NF-B) and activator protein-1 (AP-1) transcriptional factors to their recognition sites in THP-1 cells. These results suggest that serial activation of TLR1/TLR2, MyD88, the three MAPKs, and NF-B/AP-1 comprises the signaling pathways responsible for proinflammatory cytokine production by V. vulnificus IlpA. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Characterization of signaling pathways for immune response induced by a recombinant lipoprotein, IlpA of Vibrio vulnificus | - |
dc.title.alternative | 패혈증비브리오균 재조합 지질단백질 IlpA 유도 면역반응을 구성하는 면역세포 신호전달체계 규명 | - |
dc.type | Thesis | - |
dc.contributor.alternativeName | Lee, Na Yeon | - |
dc.type.local | Dissertation | - |
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