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Enhanced tumor-specific immunity of oncolytic adenovirus co-expressing IL-12 and IL-23
DC Field | Value | Language |
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dc.contributor.author | 최일규 | - |
dc.date.accessioned | 2015-12-24T08:38:39Z | - |
dc.date.available | 2015-12-24T08:38:39Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/133887 | - |
dc.description | Graduate Program for Nanomedical Science/박사 | - |
dc.description.abstract | Cytokine immunogene therapy is a promising strategy for cancer treatment. Interleukin (IL)-12 boosts potent antitumor immunity by inducing T helper 1 (Th1) cell differentiation and stimulating cytotoxic T lymphocyte (CTL) and natural killer (NK) cell cytotoxicity. IL-23 has been proposed to have similar but not overlapping functions with IL-12 in inducing Th1 cell differentiation and antitumor immunity. However, the therapeutic effects of intratumoral co-expression of IL-12 and IL-23 in a cancer model have yet to be investigated. Therefore, we investigated for the first time an effective cancer immunogene therapy of syngeneic tumors via intratumoral inoculation of oncolytic adenovirus (Ad) co-expressing IL-23 and p35, RdB/IL23/p35. Intratumoral administration of RdB/IL23/p35 elicited strong antitumor effects and increased survival in a murine B16-F10 syngeneic tumor model. The levels of IL-12, IL-23, interferon-γ (IFN-γ), and tumor necrosis factor-(TNF-) were elevated in RdB/IL23/p35-treated tumors. Moreover, the proportion of regulatory T cells was markedly decreased in mice treated with RdB/IL23/p35. Consistent with these data, mice injected with RdB/IL23/p35 showed massive infiltration of CD4+ and CD8+ T cells into the tumor as well as enhanced induction of tumor-specific immunity. Importantly, therapeutic mechanism of antitumor immune mediated by RdB/IL23/p35 is associated with the generation and recruitment of IFN-γ- and TNF--co-producing T cells in tumor microenvironment. These results provide a new insight into therapeutic mechanisms of IL-12 plus IL-23 and provide a potential clinical cancer immunotherapeutic agent for improved antitumor immunity. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Enhanced tumor-specific immunity of oncolytic adenovirus co-expressing IL-12 and IL-23 | - |
dc.title.alternative | IL-23 및 p35을 발현하는 oncolytic adenovirus의 항종양 면역효과 및 세포성 면역반응 증대 | - |
dc.type | Thesis | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04168 | - |
dc.contributor.alternativeName | Choi, Il Kyu | - |
dc.contributor.affiliatedAuthor | Choi, Il Kyu | - |
dc.type.local | Dissertation | - |
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