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Effect of trypsin on spontaneous uterine contractility in pregnant rat : possible involvement of protease-activated receptor 2
DC Field | Value | Language |
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dc.contributor.author | 김영환 | - |
dc.date.accessioned | 2015-12-24T08:34:15Z | - |
dc.date.available | 2015-12-24T08:34:15Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/133713 | - |
dc.description | Dept. of Medical Science/박사 | - |
dc.description.abstract | This study was performed to evaluate the effect of protease-activated receptor (PAR)-2 on spontaneous myometrial contraction (SMC) in isolated term pregnant myometrial strips of rat and elucidate a cellular mechanism in such an effect using conventional voltage-clamp method. In isometric tension measurement, trypsin and SL-NH2, PAR-2 agonists, significantly augmented SMC in frequency and amplitude. But, boiled trypsin (BT) and LR-NH2 had no effect on SMC. These stimulatory effects of PAR-2 agonists on SMC was nearly completely prevented by pre-application of Bay K 8644, a L-type voltage-gated Ca2+ channel activator, showing involvement of L-type voltage-gated Ca2+ channel in PAR-2-induced augmentation of SMC. In addition, PAR-2 agonists significantly enhanced L-type voltage-gated Ca2+ current (ICa-L), measured by conventional voltage-clamp method and this increase was primarily mediated by activation of phospholipase C (PLC) and protein kinase C (PKC) via G-protein activation. Taken together, we demonstrate that PAR-2 actively regulate SMC during pregnancy by modulating Ca2+ influx through L-type voltage-gated Ca2+ channel and this increase of ICa-L is primarily mediated by PLC and PKC activation. These results suggest a cellular mechanism for pathophysiological effects of PAR-2 activation on myometrical contractility during pregnancy and provide basic and theoretical information about developing new agents for the treatment of premature labor and other obstetric complication. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Effect of trypsin on spontaneous uterine contractility in pregnant rat : possible involvement of protease-activated receptor 2 | - |
dc.title.alternative | PAR-2 수용체와의 관계 | - |
dc.type | Thesis | - |
dc.contributor.alternativeName | Kim, Young Hwan | - |
dc.type.local | Dissertation | - |
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