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Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 배정호 | - |
dc.date.accessioned | 2015-12-24T08:29:11Z | - |
dc.date.available | 2015-12-24T08:29:11Z | - |
dc.date.issued | 2011 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/133520 | - |
dc.description | Dept. of Medicine/박사 | - |
dc.description.abstract | Lysozyme is a major component of airway epithelial secretions, acts as cationic antimicrobial protein for innate immunity. Although lysozyme plays an important role in airway defense and is a key component of airway secretions under inflammatory conditions, little is understood about the regulation of its expression and the associated signaling pathway. We wanted to examine whether Phorbol 12-myristate 13-acetate (PMA), one of PKC activators, treatment of the airway epithelial cell line NCI-H292 increases lysozyme gene expression. In this study, we sought to determine which signal molecules are involved in PMA-induced lysozyme gene expression. We found that PKC and mitogen activating protein/ERK2 kinase are essential for PMA-induced lysozyme expression and also mediate the PMA-induced activation of c-Myb protein. We identified a proximal region of the lysozyme promoter essential for promoter activity containing c-Myb transcription factor binding site. Additionally, by site-directed promoter mutagenesis, we identified that c-Myb preferred the CAA motif of the -85/-73 region of the lysozyme promoter. Finally, we showed that over-expression of c-Myb without PMA treatment increased the lysozyme promoter activity and protein expression. From these results, we conclude that PMA induces overexpression of lysozyme via ERK1/2 MAP kinase – c-Myb signaling pathways in NCI-H292 cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Activation of c-Myb transcription factor is critical for PMA-induced lysozyme expression in airway epithelial cells | - |
dc.title.alternative | PMA를 처치한 기도 상피 세포에서 c-Myb 전사 인자의 활성화를 통한 라이소자임의 발현 | - |
dc.type | Thesis | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학교실) | - |
dc.contributor.localId | A01806 | - |
dc.contributor.alternativeName | Bae, Jung Ho | - |
dc.contributor.affiliatedAuthor | 배정호 | - |
dc.type.local | Dissertation | - |
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