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Isolation and characterization of cancer stem cells from a heterogeneous population in human ovarian cancer
DC Field | Value | Language |
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dc.contributor.author | 고명청 | - |
dc.date.accessioned | 2015-12-24T08:26:28Z | - |
dc.date.available | 2015-12-24T08:26:28Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/133416 | - |
dc.description | Dept. of Medical Science/박사 | - |
dc.description.abstract | [한글] [영문]Cancer stem cells (CSCs) have been identified in solid tumors and cancer cell lines. Here, we isolated a series of cancer cell clones, which were heterogeneous in growth rate, cell cycle distribution, and expression profile of genes and proteins, from ovarian tumor specimens of a patient and identified a subpopulation enriched for ovarian CSCs defined by CD24 phenotype. Experiments in vitro demonstrated CD24+ subpopulation possessed stem cell-like characteristics of remaining quiescence and more chemoresistance compared to CD24- fraction, as well as a specific capacity for self-renewal and differentiation. In addition, injection of 5×103 CD24+ cells was able to form tumor xenografts in nude mice, whereas equal number of CD24- cells remained nontumorigenic. We also found CD24+ cells expressed higher mRNA levels of some “stemness” genes, including Nestin, β-catenin, Bmi-1, Oct4, Oct3/4, Notch1, and Notch4 which were involved in modulating many functions of stem cells, and lower E-cadherin mRNA level than CD24- cells. Altogether, these observations suggest human ovarian tumor cells are organized as a hierarchy and CD24 demarcates an ovarian cancer-initiating cell population. These findings will have important clinical applications for developing effective therapeutic strategies to treat ovarian cancer. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Isolation and characterization of cancer stem cells from a heterogeneous population in human ovarian cancer | - |
dc.title.alternative | 사람 난소암의 heterogenous population으로부터 종양줄기세포의 분리 및 동정 | - |
dc.type | Thesis | - |
dc.contributor.alternativeName | Gao, Ming Qing | - |
dc.type.local | Dissertation | - |
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