Effects of sulfur and lithium on blood alcohol level in rabbits
Authors
민성길
Department
Dept. of Psychiatry (정신과학교실)
Issue Date
1971
Description
의학과/석사
Abstract
[한글]
Effects of Sulfur and Lithium on Blood Alcohol Level in Rabbits
Sung Kil Min
Department of Medical Science, Graduate School, Yonsei University
In recent year, it has been known that some simple elements have marked effects
as a psychotropic drug. Lithium among others, is now considered to be an extremely
promising and intriguing drug in the treatment of mania and other psychotic
excitements. Sulfur, which is also an element, has been known as an important
component of enzymes and other organic substances. It was once need in the
pyretotherapy of dementia praecox and general paresis. And sulfur was known to
cause some calming effects, drowsiness, narcosis and central motor depression.
It has been lately reported that chlorpremazine, lithium ion, and several other
psychotropic drugs elevated the blood alcohol level in rabbits. In view of these
findings, the author conducted an animal experiment to investigate the effects of
sulfur, or in combination with lithium ion, on blood alcohol level in rabbits.
Material and Method
1. Experimental work was done with mature rabbits of both sexes, weighing about
2.0kg.
2. The experimental animals were divided into two groups; control and
experimental group, control group was given alcohol only, and experimental group
was divided into three groups; alcohol+sulfur group, alcohol+lithium group and
alcohol+lithium+sulfur group.
3. Sublimed sulfur capsule was given orally. alcohol+sulfur group and
alcohol+lithium+sulfur group were divided into three subgroups. To the 1st subgroup
sulfur was given 100mg/kg of body weight daily for 5 days, to the 2nd subgroup,
100mg/kg of body weight daily for 10 days, and to the 3rd subgroup, 200mg/kg of
body weight daily for 5 days. The last dose of sulfur was given 1.5 hour before
alcohol administration.
4. Lithium chloride solution, 6.36%, was given in a dose of 3.0mEq/kg of body
weight daily for 4 days by intravenous route.
The last dose was given 1 hour before alcohol administration.
5. In all groups, 20 vol. % ethanol solution was given in a dose of 5.0ml/kg of
body weight in 5 minutes by intravenous route.
6. All of the blood specimens were obtained by cardiac puncture at 15 and 45
minutes after alcohol administration.
7. the blood alcohol level was determined by Cavett's method.
Results
1. Alcohol+sulfur group.
In 1st subgroup, sulfur elevated the blood alcohol level at 15 minutes and
lowered at 45 minutes after alcohol administration. but those results were not
statistically significant. (p>0.05 in both) In both 2nd and 3rd subgroups, sulfur
elevated the blood alcohol level significantly at 15 minutes after alcohol
administration, (P<0.01) but not at 45 minutes.(P>0.05)
2. Alcohol+lithium group
Lithium ion elevated the blood alcohol level significantly at both 15 and 45
minutes after alcohol administration. (P<0.01 and P<0.05 respectively)
3. alcohol+lithium+sulfur group.
In all subgroups, sulfur combined with lithium ion elevated significantly the
blood alcohol level at both 15 and 45 minutes after alcohol administration.
(P<0.01) The blood alcohol levels of these subgroups were significantly higher than
those of all subgroups of alcohol+sulfur group and alcohol+lithium group.(P<0.01)
Conclusions
1. The orally administrated sublimed sulfur in a dose of 100 mg/kg/day for 5 days
had no significant effect on blood alcohol level in rabbits. But sulfur in a dose
of 100 mg/kg/day for 10 days and in a dose of 200 mg/kg/day for 5 days elevated
significantly the blood alcohol level in rabbits at 15 minutes, but not at 45
minutes after alcohol administration.
2. The intravenous injection of lithium chloride in a dose of 3 mEq/kg/day for 4
days elevated significantly the blood alcohol level in rabbits at both 15 and 45
minutes after alcohol administration.
3. The sulfur combined with lithium chloride elevated significantly the blood
alcohol level in rabbits at both 15 and 45 minutes after alcohol administration.
[영문]
In recent year, it has been known that some simple elements have marked effects as a psychotropic drug. Lithium among others, is now considered to be an extremely promising and intriguing drug in the treatment of mania and other psychotic excitements. Sulfur, which is also an element, has been known as an important
component of enzymes and other organic substances. It was once need in the pyretotherapy of dementia praecox and general paresis. And sulfur was known to cause some calming effects, drowsiness, narcosis and central motor depression.
It has been lately reported that chlorpremazine, lithium ion, and several other psychotropic drugs elevated the blood alcohol level in rabbits. In view of these findings, the author conducted an animal experiment to investigate the effects of sulfur, or in combination with lithium ion, on blood alcohol level in rabbits.
Material and Method
1. Experimental work was done with mature rabbits of both sexes, weighing about 2.0kg.
2. The experimental animals were divided into two groups; control and experimental group, control group was given alcohol only, and experimental group was divided into three groups; alcohol+sulfur group, alcohol+lithium group and alcohol+lithium+sulfur group.
3. Sublimed sulfur capsule was given orally. alcohol+sulfur group and alcohol+lithium+sulfur group were divided into three subgroups. To the 1st subgroup sulfur was given 100mg/kg of body weight daily for 5 days, to the 2nd subgroup, 100mg/kg of body weight daily for 10 days, and to the 3rd subgroup, 200mg/kg of
body weight daily for 5 days. The last dose of sulfur was given 1.5 hour before alcohol administration.
4. Lithium chloride solution, 6.36%, was given in a dose of 3.0mEq/kg of body
weight daily for 4 days by intravenous route.
The last dose was given 1 hour before alcohol administration.
5. In all groups, 20 vol. % ethanol solution was given in a dose of 5.0ml/kg of body weight in 5 minutes by intravenous route.
6. All of the blood specimens were obtained by cardiac puncture at 15 and 45 minutes after alcohol administration.
7. the blood alcohol level was determined by Cavett's method.
Results
1. Alcohol+sulfur group.
In 1st subgroup, sulfur elevated the blood alcohol level at 15 minutes and lowered at 45 minutes after alcohol administration. but those results were not statistically significant. (p>0.05 in both) In both 2nd and 3rd subgroups, sulfur elevated the blood alcohol level significantly at 15 minutes after alcohol administration, (P<0.01) but not at 45 minutes.(P>0.05)
2. Alcohol+lithium group
Lithium ion elevated the blood alcohol level significantly at both 15 and 45 minutes after alcohol administration. (P<0.01 and P<0.05 respectively)
3. alcohol+lithium+sulfur group.
In all subgroups, sulfur combined with lithium ion elevated significantly the blood alcohol level at both 15 and 45 minutes after alcohol administration.
(P<0.01) The blood alcohol levels of these subgroups were significantly higher than those of all subgroups of alcohol+sulfur group and alcohol+lithium group.(P<0.01)
Conclusions
1. The orally administrated sublimed sulfur in a dose of 100 mg/kg/day for 5 days had no significant effect on blood alcohol level in rabbits. But sulfur in a dose of 100 mg/kg/day for 10 days and in a dose of 200 mg/kg/day for 5 days elevated
significantly the blood alcohol level in rabbits at 15 minutes, but not at 45 minutes after alcohol administration.
2. The intravenous injection of lithium chloride in a dose of 3 mEq/kg/day for 4 days elevated significantly the blood alcohol level in rabbits at both 15 and 45 minutes after alcohol administration.
3. The sulfur combined with lithium chloride elevated significantly the blood alcohol level in rabbits at both 15 and 45 minutes after alcohol administration.