혈액형 ABH항원과 Secretor Gene이 비점막 선조직의 Lectin 반응에 미치는 영향

Abstract

[한글]

체액으로 분비되는 혈액형특이 물질은 secretor gene(Se, se)에 의해 좌우되므로 분비조직에서의 혈액형특이 렉틴반응에서는 Secretor 존재여부를 판별하여야만 합리적인 결과해석이 가능하다. 현재 유전자 Se의 존재는 Lewis 혈액형과 타액검사에 의해 알 수 있고 혈액형에 따라 분비되는 특이물질이 선조직내에 존재하기 위해서는 반드시 secretor gene이 있어야 가능하기 때문에 각 혈액형 뿐만 아니라 secretor 상태는 선조직의 렉틴반응에 영향을 미치는 중요한 인자가 될 수 있다. 따라서 연구자는 비점막 선조직에서 혈액형특이 렉틴인 UEA-1, DBA 및 GSI-B^^4, 와 비특이 렉틴인 SBA, WGA, RCA-1, PNA 및 Con-A를 이용하여 혈액형 ABH 항원과 se-cretor상태가 비점막 선조직의 렉틴반응에 미치는 영향을 알아보고자 본 연구를 시행하여 다음과 같은 결과를 얻었다.

1) 실험대상 85명중 65명이 secretor였으며 20명이 non-secretor였다.

2) DBA는 혈액형 A 및 AB secretor의 점액선에서만 양성반응이 다양하게 나타났다.

3) GSI-B^^4는 혈액형 B 및 AB secretor의 점액선에서만 양성반응이 다양하게 나타났다.

4) UEA-1은 모든 혈액형의 secretor의 장액선 및 점액선에서 다양한 양성반응을 보였다.

5) 혈액형비특이 물질로 알려진 SBA는 혈액형 A 및 AB secretor의 점액선에서만 양성반응이 나타났다.

6) WGA는 점액선과 장액선에, RCA틀은 점액선에서만 양성반응이 나타났으나 혈액형과 secretor 상태와는 무관하였다.

7) PNA는 혈액형과 secretor 상태에 무관하게 점액선 및 장액선에서 양성반응이 관찰되지 않았다.

8) Con-A는 장액선에만 양성반응이 나타났으며 혈액형과 secretor 상태와는 무관하였다.

본 연구의 결과로 보아 사람의 비점막에서는 혈액형과 secretor 상태에 따라 같은 조직에서도 렉틴반응이 다르게 나타날 수 있기 때문에 렉틴연구와 그 결과를 해석할 때는 반드시 이를 고려하여야 하겠다. 그 외에도 조직의 염증, 선조직의 성숙도등의 요소도 함께

고려하여야 할 것으로 사료되었다.

Lectin-binding patterns by ABO blood group and the status of secreter in human

nasal glands

Hoon Sang Chang

Department of Medical Science, The Graduate School Yonsei University

(Directed by Professor In Yong Park)

Previous study revealed a variable result of lectin-binding Patterns in the nasal

gland and this variability might be resulted from not taking account the ABO blood

group and the states of secreter. This study was designed to observe pectin-binding

patterns of the human nasal gland according to the ABO blood group and the status

of secretor. A total of 85 specimens were obtained from 85 patients who underwent

the intranasal ethmoidectomy or septoplasty. The status of secretor was examined

with Lewis ab antigen and saliva test. All specimens were fixed in 10% buffered

formalin and embedded in paraffin. Serial sections with 4㎛ thickness were made and

8 sections from each specimen were stained with blood group specific lectins(DBA,

GSI-B^^4,, UEA-I) and non-specific lectins(SBA, PNA, RCA-1, WGA, Con-A) using ABC

method. Diaminobezidine tetrahydrochloride(0.05%) was used as a chromogen. The

slides were counterstained with Mayer's hematoxylin. pectin binding patterns were

observed using a Vanox-S light microscope and following results were obtained;

1) Sixty five was secretor and twenty was non-secretor among a total of 85

subjects.

2) DBA was labelled only in mucous secretory cells of the blond group A and AB

secretor, with various degrees, ranging from a weak reaction to a strong positive.

Most of blood group A & AB non-secretor showed no positive reaction. Blood group B

and 0 showed no positive reaction in all of the cases, regardless of the secretor

state.

3) GSI-B^^4 was labelled only in mucous secretory cells of the blood group B and

AB secretor with a weak to moderate reaction. The costive reactions were mostly

heterogenous and in blood group A and 0, there was no positive reaction regardless

of the secretor state.

4) UEA-I was labelled in mucous and sersus cells of blood group A, B, AB and 0

secretor ranging from a weak to a moderte retraction. In the non-secretor, no

reaction was observed in the mucous and serous secretory cells, regardless of ABO

blood group.

5) SBA reaction was Positive only in mucous secretory cells of the blood group A

and AB secretor in various degrees from a weak to a moderate reaction. In most of

the blood group A & A8 non-secretor, no Positive reaction was observed and in the

blood group B and 0, there was no positive reaction in any of the cases, regardless

of the secretor state.

6) WGA was labelled in mucous and serous cells, irrespective of ABO blood group

and secretor state. RCA-I was labelled only in mucous cells, regardles of ABO blood

group and secretor state.

7) PNA was not labelled in any secretory cells, regardless of ABO blood group and

secretor state.

8) Con-A was labelled only in serous cells, regardless of ABO blood group and

secretor state.

These results suggest that there are blood group specific substances in human

nasal glands and their distribution was influenced by ABO blood group and the

secretor state. The maturity of nasal glands and tissue inflammation might be

another important factors to be considered in interpreting the results of section

binding Patterns in human nasal glands.

[영문]

Previous study revealed a variable result of lectin-binding Patterns in the nasal gland and this variability might be resulted from not taking account the ABO blood group and the states of secreter. This study was designed to observe pectin-binding patterns of the human nasal gland according to the ABO blood group and the status of secretor. A total of 85 specimens were obtained from 85 patients who underwent the intranasal ethmoidectomy or septoplasty. The status of secretor was examined

with Lewis ab antigen and saliva test. All specimens were fixed in 10% buffered formalin and embedded in paraffin. Serial sections with 4㎛ thickness were made and 8 sections from each specimen were stained with blood group specific lectins(DBA,

GSI-B^^4,, UEA-I) and non-specific lectins(SBA, PNA, RCA-1, WGA, Con-A) using ABC method. Diaminobezidine tetrahydrochloride(0.05%) was used as a chromogen. The

slides were counterstained with Mayer's hematoxylin. pectin binding patterns were observed using a Vanox-S light microscope and following results were obtained;

1) Sixty five was secretor and twenty was non-secretor among a total of 85 subjects.

2) DBA was labelled only in mucous secretory cells of the blond group A and AB secretor, with various degrees, ranging from a weak reaction to a strong positive.

Most of blood group A & AB non-secretor showed no positive reaction. Blood group B and 0 showed no positive reaction in all of the cases, regardless of the secretor state.

3) GSI-B^^4 was labelled only in mucous secretory cells of the blood group B and AB secretor with a weak to moderate reaction. The costive reactions were mostly heterogenous and in blood group A and 0, there was no positive reaction regardless of the secretor state.

4) UEA-I was labelled in mucous and sersus cells of blood group A, B, AB and 0 secretor ranging from a weak to a moderte retraction. In the non-secretor, no reaction was observed in the mucous and serous secretory cells, regardless of ABO blood group.

5) SBA reaction was Positive only in mucous secretory cells of the blood group A and AB secretor in various degrees from a weak to a moderate reaction. In most of the blood group A & A8 non-secretor, no Positive reaction was observed and in the blood group B and 0, there was no positive reaction in any of the cases, regardless of the secretor state.

6) WGA was labelled in mucous and serous cells, irrespective of ABO blood group and secretor state. RCA-I was labelled only in mucous cells, regardles of ABO blood group and secretor state.

7) PNA was not labelled in any secretory cells, regardless of ABO blood group and secretor state.

8) Con-A was labelled only in serous cells, regardless of ABO blood group and secretor state.

These results suggest that there are blood group specific substances in human nasal glands and their distribution was influenced by ABO blood group and the secretor state. The maturity of nasal glands and tissue inflammation might be

another important factors to be considered in interpreting the results of section binding Patterns in human nasal glands.