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저분자량 키토산/유전자 나노콤플렉스 제조 및 이를 이용한 293 세포로의 전달
DC Field | Value | Language |
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dc.contributor.author | 장양수 | - |
dc.date.accessioned | 2015-08-26T16:43:33Z | - |
dc.date.available | 2015-08-26T16:43:33Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0304-128X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114976 | - |
dc.description.abstract | Synthetic gene carriers such as poly-cationic polymers easily form complexes with plasmid DNA which contains negative charge. Chitosan is a polysaccharide that demonstrates much potential as a gene delivery system. The ability of depolymerized chitosan to condense DNA was determined using electrophoresis. Dynamic laser scattering and scanning electron microscopy were used to examine the size and the morphology of the chitosan/DNA complex. Parameters such as chitosan molecular weight and charge density influenced the complex size and the DNA amount condensed with chitosan. The cell viabilities in the presence of chitosan ranged between 84-108% of the control in all experiments. Gene expression efficacy using chitosan/DNA complex was enhanced in 293 cells relative to that using naked DNA, although it was lower than that using lipofecamine. Transfection efficacy using low molecular weight chitosan (Mw=8,517) was higher than those of the control and the other chitosan (MW=4,078). The low molecular weight chitosan (MW=8,517) with a high charge density (18.32 mV) fulfilled the requirements for a suitable model gene delivery system with respect to the condensing ability of DNA, complex formation, and transfection efficacy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 313~317 | - |
dc.relation.isPartOf | Korean Chemical Engineering Research (화학공학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Gene Delivery | - |
dc.subject.MESH | Chitosan | - |
dc.subject.MESH | Molecular Weight | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Nano Complex | - |
dc.title | 저분자량 키토산/유전자 나노콤플렉스 제조 및 이를 이용한 293 세포로의 전달 | - |
dc.title.alternative | DNA Condensation and Delivery in 293 Cells Using Low Molecular Weight Chitosan/gene Nano-complex | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | 방시원 | - |
dc.contributor.googleauthor | 장양수 | - |
dc.contributor.googleauthor | 김중현 | - |
dc.contributor.googleauthor | 김우식 | - |
dc.identifier.doi | OAK-2005-02245 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03448 | - |
dc.relation.journalcode | J01951 | - |
dc.subject.keyword | Gene Delivery | - |
dc.subject.keyword | Chitosan | - |
dc.subject.keyword | Molecular Weight | - |
dc.subject.keyword | Transfection | - |
dc.subject.keyword | Nano Complex | - |
dc.contributor.alternativeName | Jang, Yang Soo | - |
dc.contributor.affiliatedAuthor | Jang, Yang Soo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 43 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 313 | - |
dc.citation.endPage | 317 | - |
dc.identifier.bibliographicCitation | Korean Chemical Engineering Research (화학공학회지), Vol.43(2) : 313-317, 2005 | - |
dc.identifier.rimsid | 39354 | - |
dc.type.rims | ART | - |
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