Cited 280 times in
Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3 beta is required for adipogenesis
DC Field | Value | Language |
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dc.contributor.author | 김재우 | - |
dc.date.accessioned | 2015-08-26T16:43:21Z | - |
dc.date.available | 2015-08-26T16:43:21Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114970 | - |
dc.description.abstract | CCAAT enhancer-binding protein (C/EBP)β, C/EBPα, and peroxisome proliferator activated receptor (PPAR)γ act in a cascade where C/EBPβ activates expression of C/EBPα and PPARγ, which then function as pleiotropic activators of genes that produce the adipocyte phenotype. When growth-arrested 3T3-L1 preadipocytes are induced to differentiate, C/EBPβ is rapidly expressed but still lacks DNA-binding activity. After a long (14-hour) lag, glycogen synthase kinase 3β enters the nucleus, which correlates with hyperphosphorylation of C/EBPβ and acquisition of DNA-binding activity. Concurrently, 3T3-L1 preadipocytes synchronously enter S phase and undergo mitotic clonal expansion, a prerequisite for terminal differentiation. Ex vivo and in vitro experiments with C/EBPβ show that phosphorylation of Thr-188 by mitogen-activating protein kinase “primes” C/EBPβ for subsequent phosphorylation on Ser-184 and Thr-179 by glycogen synthase kinase 3β, acquisition of DNA-binding function, and transactivation of the C/EBPα and PPARγ genes. The delayed transactivation of the C/EBPα and PPARγ genes by C/EBPβ appears necessary to allow mitotic clonal expansion, which would otherwise be prevented, because C/EBPα and PPARγ are antimitotic. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 9766~9771 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | 3T3-L1 Cells | - |
dc.subject.MESH | Adipocytes/cytology* | - |
dc.subject.MESH | Adipocytes/metabolism | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | CCAAT-Enhancer-Binding Protein-beta/metabolism* | - |
dc.subject.MESH | Cell Differentiation/physiology* | - |
dc.subject.MESH | Cell Nucleus/metabolism | - |
dc.subject.MESH | Chromatin Immunoprecipitation | - |
dc.subject.MESH | Chromatography, Liquid | - |
dc.subject.MESH | Electrophoresis, Polyacrylamide Gel | - |
dc.subject.MESH | Glycogen Synthase Kinase 3/metabolism* | - |
dc.subject.MESH | Glycogen Synthase Kinase 3 beta | - |
dc.subject.MESH | Immunoblotting | - |
dc.subject.MESH | Isoelectric Focusing | - |
dc.subject.MESH | Mass Spectrometry | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases/metabolism* | - |
dc.subject.MESH | PPAR gamma/metabolism | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Transcriptional Activation/physiology | - |
dc.title | Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3 beta is required for adipogenesis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Qi-Qun Tang | - |
dc.contributor.googleauthor | Mads Grønborg | - |
dc.contributor.googleauthor | M. Daniel Lane | - |
dc.contributor.googleauthor | Akhilesh Pandey | - |
dc.contributor.googleauthor | Tamara C. Otto | - |
dc.contributor.googleauthor | Jae-Woo Kim | - |
dc.contributor.googleauthor | Haiyan Huang | - |
dc.identifier.doi | 10.1073/pnas.0503891102 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00865 | - |
dc.relation.journalcode | J02550 | - |
dc.identifier.eissn | 1091-6490 | - |
dc.identifier.pmid | 15985551 | - |
dc.subject.keyword | 3T3-L1 preadipocyte | - |
dc.subject.keyword | cell cycle | - |
dc.subject.keyword | differentiation | - |
dc.subject.keyword | mitotic clonal expansion | - |
dc.contributor.alternativeName | Kim, Jae Woo | - |
dc.contributor.affiliatedAuthor | Kim, Jae Woo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 102 | - |
dc.citation.number | 28 | - |
dc.citation.startPage | 9766 | - |
dc.citation.endPage | 9771 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.102(28) : 9766-9771, 2005 | - |
dc.identifier.rimsid | 39349 | - |
dc.type.rims | ART | - |
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