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Role of the hindbrain in dorsoventral but not anteroposterior axial specification of the inner ear

Authors
 Jinwoong Bok  ;  Marianne Bronner-Fraser  ;  Doris K. Wu 
Citation
 DEVELOPMENT, Vol.132(9) : 2115-2124, 2005 
Journal Title
 DEVELOPMENT 
ISSN
 0950-1991 
Issue Date
2005
MeSH
Animals ; Chick Embryo ; Ear, Inner/embryology* ; Ear, Inner/physiology ; Embryonic Induction/physiology* ; Hedgehog Proteins ; Notochord/metabolism ; Rhombencephalon/physiology* ; Trans-Activators/physiology
Keywords
Inner ear ; Axis ; Axial specification ; Induction ; Hindbrain ; Rhombomere ; Sonic hedgehog ; SHH ; Chicken
Abstract
An early and crucial event in vertebrate inner ear development is the acquisition of axial identities that in turn dictate the positions of all subsequent inner ear components. Here, we focus on the role of the hindbrain in establishment of inner ear axes and show that axial specification occurs well after otic placode formation in chicken. Anteroposterior (AP) rotation of the hindbrain prior to specification of this axis does not affect the normal AP orientation and morphogenesis of the inner ear. By contrast, reversing the dorsoventral (DV) axis of the hindbrain results in changing the DV axial identity of the inner ear. Expression patterns of several ventrally expressed otic genes such as NeuroD, Lunatic fringe (Lfng) and Six1 are shifted dorsally, whereas the expression pattern of a normally dorsal-specific gene, Gbx2, is abolished. Removing the source of Sonic Hedgehog (SHH) by ablating the floor plate and/or notochord, or inhibiting SHH function using an antibody that blocks SHH bioactivity results in loss of ventral inner ear structures. Our results indicate that SHH, together with other signals from the hindbrain, are important for patterning the ventral axis of the inner ear. Taken together, our studies suggest that tissue(s) other than the hindbrain confer AP axial information whereas signals from the hindbrain are necessary and sufficient for the DV axial patterning of the inner ear.
Files in This Item:
T200504585.pdf Download
DOI
10.1242/dev.01796
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Bok, Jin Woong(복진웅) ORCID logo https://orcid.org/0000-0003-1958-1872
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114956
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