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Protection of human fibroblasts from reactive oxygen species by green tea polyphenolic compounds

 Dong Wook Han  ;  H.H. Kim  ;  Jong Chul Park  ;  Suong Hyu Hyon  ;  Kwon Yong Lee  ;  Hyun Sook Baek  ;  Hyun Joo Son 
 Key Engineering Materials, Vol.288~289 : 665-668, 2005 
Journal Title
 Key Engineering Materials 
Issue Date
Green Tea Polyphenolic Compounds ; Human Dermal Fibroblast ; Oxidative Stress ; Viability
Green Tea Polyphenolic Compounds ; Human Dermal Fibroblast ; Oxidative Stress ; Viability
The potential protective roles played by green tea compounds (GTPCs) against reactive oxygen species-induced oxidative stress in cultured fetal human dermal fibroblasts (fHDFs) were investigated according to cell viability measurement methods, such as fluorescence double staining followed by flow cytometry (FCM), MTT assay and crystal violet uptake. Oxidative stress was induced in the fHDFs, either by adding 50 mM H2O2 or by the action of 40 U/L xanthine oxidase (XO) in the presence of xanthine (250 µM). FCM analysis was the most suitable to show that both treatments produced a significant (p < 0.05) reduction in the fHDF viability, attributed to its high sensitivity. On the microscopic observations, the cell death with necrotic morphology was appreciably induced by both treatments. These oxidative stress-induced damages were significantly (p < 0.05) prevented by pre-incubating the fHDFs with 200 µg/ml GTPC for 1 h. These results suggest that GTPC can act as a biological antioxidant in a cell culture experimental model and prevent oxidative stress-induced cytotoxicity in cells.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kim, Hak Hee(김학희)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Baek, Hyun Sook(백현숙)
Son, Hyun Joo(손현주)
Han, Dong Wook(한동욱)
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