Cited 9 times in
Rapid blockade of telomerase activity and tumor cell growth by the DPL lipofection of ribbon antisense to hTR
DC Field | Value | Language |
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dc.contributor.author | 이윤한 | - |
dc.date.accessioned | 2015-08-26T16:36:45Z | - |
dc.date.available | 2015-08-26T16:36:45Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114788 | - |
dc.description.abstract | Ribbon antisense (RiAS) to the hTR RNA, a component of the telomerase complex, was employed to inhibit telomerase activity and cancer cell growth. The antisense molecule, hTR-RiAS, combined with enhanced cellular uptake was shown to effectively inhibit telomerase activity and cause rapid cell death in various cancer cell lines. When cancer cells were treated with hTR-RiAS, the level of hTR RNA was reduced by more than 90% accompanied with reduction in telomerase activity. When checked for cancer cell viability, cancer cell lines treated with hTR-RiAS using DNA+Peptide+Lipid complex showed 70–80% growth inhibition in 3 days. The reduced cell viability was due to apoptosis as the percentage of cells exhibiting the sub-G0 arrest and DNA fragmentation increased after antisense treatment. Further, when subcutaneous tumors of a colon cancer cell line (SW480) were treated intratumorally with hTR-RiAS, tumor growth was markedly suppressed with almost total ablation of hTR RNA in the tumor tissue. Cells in the tumor tissue were also found to undergo apoptosis after hTR-RiAS treatment. These results suggest that hTR-RiAS is an effective anticancer reagent, with a potential for broad efficacy to diverse malignant tumors. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 6492~6501 | - |
dc.relation.isPartOf | ONCOGENE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Drug Screening Assays, Antitumor/methods | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Liposomes/administration & dosage* | - |
dc.subject.MESH | Liposomes/chemistry | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Neoplasms, Experimental/drug therapy | - |
dc.subject.MESH | Neoplasms, Experimental/pathology | - |
dc.subject.MESH | RNA, Antisense/genetics | - |
dc.subject.MESH | RNA, Antisense/pharmacology* | - |
dc.subject.MESH | Telomerase/antagonists & inhibitors* | - |
dc.subject.MESH | Telomerase/genetics | - |
dc.subject.MESH | Telomerase/metabolism | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.title | Rapid blockade of telomerase activity and tumor cell growth by the DPL lipofection of ribbon antisense to hTR | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | Arun K Bajpai | - |
dc.contributor.googleauthor | Jeong-Hoh Park | - |
dc.contributor.googleauthor | Jong-Gu Park | - |
dc.contributor.googleauthor | Byeong-Churl Chang | - |
dc.contributor.googleauthor | Seong-Il Suh | - |
dc.contributor.googleauthor | Kyung-Oh Doh | - |
dc.contributor.googleauthor | Yun-Han Lee | - |
dc.contributor.googleauthor | Hyungu Kang | - |
dc.contributor.googleauthor | Ik-Jae Moon | - |
dc.identifier.doi | 10.1038/sj.onc.1208731 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03029 | - |
dc.relation.journalcode | J02413 | - |
dc.identifier.eissn | 1476-5594 | - |
dc.identifier.pmid | 16170384 | - |
dc.identifier.url | http://www.nature.com/onc/journal/v24/n43/full/1208731a.html | - |
dc.subject.keyword | telomerase | - |
dc.subject.keyword | hTR | - |
dc.subject.keyword | ribbon antisense | - |
dc.subject.keyword | DPL transfection | - |
dc.contributor.alternativeName | Lee, Yun Han | - |
dc.contributor.affiliatedAuthor | Lee, Yun Han | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 24 | - |
dc.citation.number | 43 | - |
dc.citation.startPage | 6492 | - |
dc.citation.endPage | 6501 | - |
dc.identifier.bibliographicCitation | ONCOGENE, Vol.24(43) : 6492-6501, 2005 | - |
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