Cited 141 times in
SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran.
DC Field | Value | Language |
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dc.contributor.author | 박채규 | - |
dc.date.accessioned | 2015-07-15T17:19:55Z | - |
dc.date.available | 2015-07-15T17:19:55Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0953-8178 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114691 | - |
dc.description.abstract | The marginal zone macrophages of the spleen are implicated in the clearance of polysaccharides, but underlying mechanisms need to be pinpointed. SIGN-R1 is one of five recently identified mouse genes that are homologous to human DC-SIGN and encode a single, external, C-terminal C-type lectin domain. We find that a polyclonal antibody to a specific SIGN-R1 peptide reacts primarily and strongly with a subset of macrophages in the marginal zone of spleen and lymph node medulla. In both sites, SIGN-R1 exists primarily in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. Upon transfection into three different cell lines, high-mol.-wt forms bearing SIGN-R1 are expressed, as well as reactivity with ER-TR9, a mAb previously described to react selectively with marginal zone macrophages. SIGN-R1-expressing macrophages preferentially sequester dextrans following i.v. injection. Likewise, when phagocytic cells are enriched from spleen and tested in culture, dextran is selectively endocytosed by a subset of very large SIGN-R1(+) cells representing approximately 5% of total released macrophages. Uptake of FITC-dextran by these macrophages in vivo and in vitro is blocked by ER-TR9 and polyclonal anti-SIGN-R1 antibodies. Following transfection with SIGN-R1, cell lines become competent to endocytose dextrans. The dextran localizes primarily to compartments lacking transferrin receptor and the LAMP-1 CD107a panlysosomal antigen. Therefore, SIGN-R1 mediates the uptake of dextran polysaccharides, and it is predominantly expressed in the macrophages of the splenic marginal zone and lymph node medulla. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 177~186 | - |
dc.relation.isPartOf | INTERNATIONAL IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Adhesion Molecules/genetics | - |
dc.subject.MESH | Dextrans/metabolism* | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Lectins, C-Type/genetics* | - |
dc.subject.MESH | Lectins, C-Type/metabolism | - |
dc.subject.MESH | Lymph Nodes/metabolism | - |
dc.subject.MESH | Macrophages/metabolism* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Receptors, Cell Surface/genetics | - |
dc.subject.MESH | Spleen/metabolism* | - |
dc.title | SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Life Science (의생명과학부) | - |
dc.contributor.googleauthor | Young‐Sun Kang | - |
dc.contributor.googleauthor | Sayuri Yamazaki | - |
dc.contributor.googleauthor | Chae Gyu Park | - |
dc.contributor.googleauthor | Ralph M. Steinman | - |
dc.contributor.googleauthor | Kayo Inaba | - |
dc.contributor.googleauthor | Kazuhiko Takahara | - |
dc.contributor.googleauthor | Jae Y. Kim | - |
dc.contributor.googleauthor | Sandra A. Bruening | - |
dc.contributor.googleauthor | Maggie Pack | - |
dc.contributor.googleauthor | Tomonori Iyoda | - |
dc.identifier.doi | 10.1093/intimm/dxg019 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01718 | - |
dc.relation.journalcode | J01080 | - |
dc.identifier.eissn | 1460-2377 | - |
dc.identifier.pmid | 12578847 | - |
dc.subject.keyword | dextran | - |
dc.subject.keyword | endocytosis | - |
dc.subject.keyword | macrophage | - |
dc.subject.keyword | marginal zone | - |
dc.subject.keyword | SIGN-R1 | - |
dc.contributor.alternativeName | Park, Chae Gyu | - |
dc.contributor.affiliatedAuthor | Park, Chae Gyu | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 15 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 177 | - |
dc.citation.endPage | 186 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL IMMUNOLOGY, Vol.15(2) : 177-186, 2003 | - |
dc.identifier.rimsid | 46063 | - |
dc.type.rims | ART | - |
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