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Site-directed Mutations in the Tumor-associated Cytokine, Autotaxin, Eliminate Nucleotide Phosphodiesterase, Lysophospholipase D, and Motogenic Activities
DC Field | Value | Language |
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dc.contributor.author | 고은진 | - |
dc.date.accessioned | 2015-07-15T17:19:44Z | - |
dc.date.available | 2015-07-15T17:19:44Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114686 | - |
dc.description.abstract | The exo-enzyme autotaxin/NPP2 (ATX/NPP2) is a potent stimulator of cell migration, invasion, metastasis, and angiogenesis. Recently, ATX/NPP2 was found to possess lysophospholipase D (lyso-LPD) activity, generating the bioactive mediator lysophosphatidic acid from precursors. In the present study, we used site-directed mutagenesis to delineate the active domain of lysophospholipid catalytic activity and to examine potential overlap with the nucleotide phosphodiesterase domain. We found four amino acid residues obligatory for the phosphodiesterase, lyso-PLD, and migration-stimulating activities of ATX/NPP2, suggesting that 5'-nucleotide phosphodiesterase (PDE) and lyso-PLD share a common reaction mechanism and inviting design of enzymatic inhibitors as therapeutic agents for neoplastic disease. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | CANCER RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | COS Cells | - |
dc.subject.MESH | Cell Movement/genetics | - |
dc.subject.MESH | Cercopithecus aethiops | - |
dc.subject.MESH | Glucose-6-Phosphate Isomerase/genetics* | - |
dc.subject.MESH | Glucose-6-Phosphate Isomerase/metabolism* | - |
dc.subject.MESH | Glycoproteins/genetics* | - |
dc.subject.MESH | Glycoproteins/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Multienzyme Complexes* | - |
dc.subject.MESH | Mutagenesis, Site-Directed | - |
dc.subject.MESH | Phosphodiesterase I | - |
dc.subject.MESH | Phosphoric Diester Hydrolases/genetics | - |
dc.subject.MESH | Phosphoric Diester Hydrolases/metabolism* | - |
dc.subject.MESH | Point Mutation* | - |
dc.subject.MESH | Protein Structure, Tertiary | - |
dc.subject.MESH | Pyrophosphatases | - |
dc.subject.MESH | Receptors, Purinergic P1/physiology | - |
dc.subject.MESH | Structure-Activity Relationship | - |
dc.title | Site-directed Mutations in the Tumor-associated Cytokine, Autotaxin, Eliminate Nucleotide Phosphodiesterase, Lysophospholipase D, and Motogenic Activities | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Eunjin Koh | - |
dc.contributor.googleauthor | Timothy Clair | - |
dc.contributor.googleauthor | Mary Stracke | - |
dc.contributor.googleauthor | Lance Liotta | - |
dc.contributor.googleauthor | Elliott Schiffmann | - |
dc.contributor.googleauthor | Elisa C. Woodhouse | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00139 | - |
dc.relation.journalcode | J00452 | - |
dc.identifier.eissn | 1538-7445 | - |
dc.identifier.pmid | 12727817 | - |
dc.subject.keyword | 12727817 | - |
dc.contributor.alternativeName | Koh, Eun Jin | - |
dc.contributor.affiliatedAuthor | Koh, Eun Jin | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 63 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2042 | - |
dc.citation.endPage | 2045 | - |
dc.identifier.bibliographicCitation | CANCER RESEARCH, Vol.63(9) : 2042-2045, 2003 | - |
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