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Identification of a ligand for gluticorticoid-induced tumor necrosis receptor constitutively expressed in dendritic cells

Authors
 Kang-Yeol Yu  ;  Han Soo Kim  ;  Jae Jun Jeong  ;  Sung-Shik Min  ;  Si Young Song  ;  Byung-S Youn 
Citation
 Biochemical and Biophysical Research Communications, Vol.310(2) : 433-438, 2003 
Journal Title
 Biochemical and Biophysical Research Communications 
ISSN
 0006-291X 
Issue Date
2003
MeSH
Amino Acid Sequence ; Animals ; Carrier Proteins/chemistry ; Carrier Proteins/metabolism* ; Cell Line ; Cell Line, Tumor ; Dendritic Cells/immunology* ; Glucocorticoid-Induced TNFR-Related Protein ; Ligands ; Mice ; Molecular Sequence Data ; Receptors, Nerve Growth Factor/metabolism* ; Receptors, Tumor Necrosis Factor/metabolism* ; Sequence Alignment ; Tumor Necrosis Factors*
Keywords
14521928
Abstract
Glucocorticoid-induced tumor necrosis receptor (GITR) has been implicated in regulation of T cell suppression by CD25(+)CD4(+) regulatory T cells (Tregs). We isolated a cDNA encoding GITR ligand (GITRL) from mouse endothelioma cells. When stably expressed in HEK293 cells, its specific interaction with GITR was confirmed by flow cytometry with the use of GITR-Fc. The interaction was greatly diminished by the addition of soluble GITRL. Consistent with this, soluble GITRL bound to the cell surface of the GITR-expressing HEK293 cells. Coexpression of GITR with GITRL or stimulation of the GITR-expressing cells with soluble GITRL led to activation of NF-kappaB, which was significantly reduced by anti-GITR. More importantly, GITRL was expressed by both immature and mature dendritic cells, suggesting that the interaction between GITR and GITRL may contribute to immune regulation of Tregs by dendritic cells. This isolated TNFRL represents a bona fide GITRL whose presence has been elusive until this time.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X03018084
DOI
10.1016/j.bbrc.2003.09.024
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Han Soo(김한수)
Song, Si Young(송시영) ORCID logo https://orcid.org/0000-0002-1417-4314
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/114534
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