Cited 3 times in
Feasibility to Generate Monocyte-Derived Dendritic Cell from Coculture with Melanoma Tumor Cells in the Presence of Granulocyte / Macrophage Colony-Stimulating Factor (GM-CSF) and Interleukin-4
DC Field | Value | Language |
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dc.contributor.author | 김영태 | - |
dc.date.accessioned | 2015-07-15T17:10:58Z | - |
dc.date.available | 2015-07-15T17:10:58Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1046-7408 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114394 | - |
dc.description.abstract | OBJECTIVE: We investigated how melanoma cells and membrane-bound granulocyte/macrophage colony stimulating factor (mbGM-CSF) melanoma cell lines affect the differentiation of dendritic cells (DC) from CD14+ monocytes. METHOD OF STUDY: The malignant melanoma cell lines (Conley and Jorp) and mbGM-CSF-positive cell lines (Conley B-F8 and Jorp C-E6) were cultured and cell-free supernatants were collected from each cell line cultures to assess the GM-CSF level. Adherent monocytes were cocultured for 6-7 days with irradiated mbGM-CSF and wild type melanoma cells (50 Gy) at each 1:1 and 0.1:1 ratio in six-well culture plates in ex vivo culture medium containing interleukin (IL)-4. Immunophenotyping was performed by triple color immunofluorescence staining with flow cytometry analysis. RESULTS: GM-CSF was detected at low levels in the culture supernatants of Conley B-F8 (0.48 ng/10(6) cells/24 hr), whereas there was no detectable GM-CSF in that of Conley melanoma cell line. Monocytes cultured with GM-CSF/IL-4 generated the expression of high levels of HLA DR, CD1a and CD86, while the expression of CD14 and CD83 were in low amounts. However, the addition of GM-CSF to these cultures resulted in an increased expression of these markers and decreased that of CD14. Monocytes cocultured with Jorp C-E6 illustrated similar expression pattern of CD1a, HLA DR and CD14 in the presence or absence of GM-CSF as Conley B-F8 melanoma cell line. Monocytes cocultured with Conley B-F8 melanoma cells at 1:1 and 0.1:1 ratio showed no significant difference in expression of CD1a, CD14 and CD83 between the two ratios. CONCLUSION: Our results illustrate the feasibility to generate monocyte-derived DC from coculture with melanoma tumor cells in the presence of GM-CSF and IL-4. However, mbGM-CSF tumor cells did not significantly enhance the DC differentiation through juxtacrine stimulation unless soluble GM-CSF was added in the culture media. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 230~238 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | coculture | - |
dc.subject.MESH | dendritic cell | - |
dc.subject.MESH | GM-CSF | - |
dc.subject.MESH | mbGM-CSF | - |
dc.title | Feasibility to Generate Monocyte-Derived Dendritic Cell from Coculture with Melanoma Tumor Cells in the Presence of Granulocyte / Macrophage Colony-Stimulating Factor (GM-CSF) and Interleukin-4 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Obstetrics & Gynecology (산부인과학) | - |
dc.contributor.googleauthor | Young T. Kim | - |
dc.contributor.googleauthor | Evan M. Hersh | - |
dc.contributor.googleauthor | Katrina T. Trevor | - |
dc.identifier.doi | 10.1034/j.1600-0897.2003.01200.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00729 | - |
dc.relation.journalcode | J00111 | - |
dc.identifier.eissn | 1600-0897 | - |
dc.identifier.pmid | 10.1034/j.1600-0897.2003.01200.x | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1034/j.1600-0897.2003.01200.x/abstract | - |
dc.subject.keyword | coculture | - |
dc.subject.keyword | dendritic cell | - |
dc.subject.keyword | GM-CSF | - |
dc.subject.keyword | mbGM-CSF | - |
dc.contributor.alternativeName | Kim, Young Tae | - |
dc.contributor.affiliatedAuthor | Kim, Young Tae | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 49 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 230 | - |
dc.citation.endPage | 238 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Vol.49(4) : 230-238, 2003 | - |
dc.identifier.rimsid | 47275 | - |
dc.type.rims | ART | - |
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