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Methods for the study of protein-protein interactions in cancer cell biology.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박이하 | - |
dc.date.accessioned | 2015-07-15T17:10:30Z | - |
dc.date.available | 2015-07-15T17:10:30Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114378 | - |
dc.description.abstract | Development of sensitive methods to monitor and quantitatively assess the expression levels of endogenous genes and the association-interaction of proteins in living cells and whole organisms is a complex and challenging problem. In this chapter, we have described basic methods for investigating protein-protein interactions which include immunoprecipitation, GST pull-down assays, peptide bead pull-down assays, chemical crosslinking and photoaffinity labeling. These methods should provide important tools to dissect crosstalk between proteins and the direct implications of this crosstalk in signaling pathways and cancer biology | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 255~267 | - |
dc.relation.isPartOf | Methods in Molecular Biology (Clifton, N.J.) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Membrane/metabolism | - |
dc.subject.MESH | Cross-Linking Reagents | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Neoplasms/metabolism* | - |
dc.subject.MESH | Photoaffinity Labels | - |
dc.subject.MESH | Precipitin Tests/methods* | - |
dc.subject.MESH | Proteins/isolation & purification | - |
dc.subject.MESH | Proteins/metabolism* | - |
dc.subject.MESH | Receptor Cross-Talk | - |
dc.subject.MESH | Recombinant Fusion Proteins/isolation & purification | - |
dc.subject.MESH | Recombinant Fusion Proteins/metabolism | - |
dc.subject.MESH | Signal Transduction* | - |
dc.title | Methods for the study of protein-protein interactions in cancer cell biology. | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Oral Cancer Research Institute (구강종양연구소) | - |
dc.contributor.googleauthor | Daniel Price | - |
dc.contributor.googleauthor | Iha Park | - |
dc.contributor.googleauthor | Hava Avraham | - |
dc.identifier.doi | 10.1385/1-59259-356-9:255 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01623 | - |
dc.relation.journalcode | J02226 | - |
dc.identifier.pmid | 12616725 | - |
dc.identifier.url | http://link.springer.com/protocol/10.1385/1-59259-356-9:255 | - |
dc.subject.keyword | signaling | - |
dc.subject.keyword | association | - |
dc.subject.keyword | direct-interaction | - |
dc.subject.keyword | protein-protein interactions | - |
dc.subject.keyword | immunoprecipitation | - |
dc.subject.keyword | GST-binding domains | - |
dc.contributor.alternativeName | Park, Iha | - |
dc.contributor.affiliatedAuthor | Park, Iha | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 218 | - |
dc.citation.startPage | 255 | - |
dc.citation.endPage | 267 | - |
dc.identifier.bibliographicCitation | Methods in Molecular Biology (Clifton, N.J.), Vol.218 : 255-267, 2003 | - |
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