신장이식 후 1년 이상 경과한 환자의 말초혈액 단핵구에서 측정한활성산소족 농도와 혈청 크레아티닌과의 상관관계 분석

Abstract

Purpose: Oxidative stress plays an important role in the development and progression of renal injury. However, the role of reactive oxygen species (ROS) in renal allograft dysfunction is not clear. The present study examined the level of intracellular ROS in healthy control (kidney donor, n=37), end-stage renal disease (ESRD) patients (n=36), transplant recipients with serum creatinine (Scr) less than 1.5 mg% (n=33), and recipients with Scr between 1.5 and 5.0 mg% (n=36) at least one year after renal transplantation.

Methods: Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Hypaque gradient method. Dichlorofluorescein (DCF)-sensitive ROS was measured by flow cytometry and expressed as an arbitrary unit.

Results: Basal ROS production in PBMC was significantly increased in ESRD patients compared to healthy control. Basal ROS production in both transplant patient groups was not significantly different from healthy control. Phorbol-12-myristate-13- acetate (PMA) and hydrogen peroxide significantly enhanced intracellular ROS in all 4 groups. PMA- and hydrogen peroxide-induced cellular ROS was significantly higher in renal recipients with Scr between 1.5 and 5.0 mg% than in both healthy control and patients with Scr below 1.5 mg%. In regression analysis all, PMA- and hydrogen peroxide- induced as well as basal intracellular ROS in PBMC was correlated with Scr.

Conclusion: Our results demonstrate that oxidative stress correlates with the declining of renal graft function.