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8-Hydroxydeoxyguanosine Causes Death of Human Leukemia Cells Deficient in 8-Oxoguanine Glycosylase 1 Activity by Inducing Apoptosis
DC Field | Value | Language |
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dc.contributor.author | 이윤실 | - |
dc.date.accessioned | 2015-07-15T17:04:48Z | - |
dc.date.available | 2015-07-15T17:04:48Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1541-7786 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114184 | - |
dc.description.abstract | Our previous study showed that KG-1, a human acute leukemia cell line, has mutational loss of 8-oxoguanine (8-hydroxyguanine; oh(8)Gua) glycosylase 1 (OGG1) activity and that its viability is severely affected by 8-hydroxydeoxyguanosine (8-oxodeoxyguanosine; oh(8)dG). In the present study, the nature of the killing action of oh(8)dG on KG-1 was investigated. Signs observed in oh(8)dG-treated KG-1 cells indicated that death was due to apoptosis, as demonstrated by: increased sub-G(1) hypodiploid (apoptotic) cells, DNA fragmentation, and apoptotic body formation; loss of mitochondrial transmembrane potential, the release of cytochrome c from mitochondria into the cytosol, and the down-regulation of bcl-2; and the activation of caspases 8, 9, and 3, and the efficient inhibition of the apoptotic process by caspases inhibitors. This apoptosis appears not to be associated with Fas/Fas ligand because the expressions of these proteins were unchanged. Apoptotic KG-1 cells showed a high concentration of oh(8)Gua in DNA. Moreover, the increased concentration of oh(8)Gua in DNA, and the apoptotic process were not suppressed by the antioxidant, N-acetylcysteine, and thus the process is independent of reactive oxygen species. Of the 18 cancer cell lines treated with oh(8)dG, 3 cell lines (H9, CEM-CM3, and Molt-4) were found to be committed to apoptosis, and all of these showed very low OGG1 activity and a marked increase in the concentration of oh(8)Gua in DNA. These observations indicate that in addition to its mutagenic action, oh(8)Gua in DNA disturbs cell viability by inducing apoptosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | MOLECULAR CANCER RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 8-Hydroxydeoxyguanosine Causes Death of Human Leukemia Cells Deficient in 8-Oxoguanine Glycosylase 1 Activity by Inducing Apoptosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | Jin-Won Hyun | - |
dc.contributor.googleauthor | Yoon-Chul Jung | - |
dc.contributor.googleauthor | Myung-Hee Chung | - |
dc.contributor.googleauthor | Ho-Jin You | - |
dc.contributor.googleauthor | Jinhee Choi | - |
dc.contributor.googleauthor | Yun-Sil Lee | - |
dc.contributor.googleauthor | Sun-Hee Yoon | - |
dc.contributor.googleauthor | Byung-Hak Yoon | - |
dc.contributor.googleauthor | Ja-Eun Kim | - |
dc.contributor.googleauthor | Eun-Young Choi | - |
dc.contributor.googleauthor | Hyun-Sook Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03021 | - |
dc.relation.journalcode | J02253 | - |
dc.identifier.eissn | 1557-3125 | - |
dc.contributor.alternativeName | Lee, Yun Sil | - |
dc.contributor.affiliatedAuthor | Lee, Yun Sil | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 1 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 290 | - |
dc.citation.endPage | 299 | - |
dc.identifier.bibliographicCitation | MOLECULAR CANCER RESEARCH, Vol.1(4) : 290-299, 2003 | - |
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