레티노인산 결핍에 의한 사람 중이점막 상피세포의 각화편평상피세포로의 분화

Abstract

Background and Objectives：The pathogenesis of cholesteatoma behind an intact tympanic membrane remains controversial. Squamous metaplasia of the middle ear mucosa is thought to be a possible mechanism in such cases. However, to date, no definitive experimental results have proved the association. This study was undertaken to investigate whether normal human middle ear epithelial (NHMEE) cells undergo keratinizing squamous differentiation in retinoic acid (RA)-deficient culture.

Materials and Method：We examined the morphological differences between RA-deficient and RA-sufficient cultures, and determined the expressions of the mucin gene and cornifin-α mRNAs as indicators of mucous and squamous differentiation, respectively.

Results：Histomorphologically, the NHMEE cells differentiated into a keratinizing squamous epithelium in RA-deficient culture. In addition, the expressions of mucin gene 5AC (MUC5AC) and MUC8 mRNA were suppressed, and the expression of cornifin-α mRNA increased progressively as a function of differentiation in RA-deficient culture.

Conclusion：Our study shows that RA depletion induces keratinizing squamous differentiation in NHMEE cell culture.