계란 알레르기 환아에서 오리알과 거위알에 대한 교차반응

Abstract

Purpose : Birds' eggs have been a major source of food for mankind since the time unknown.. Among them, chicken eggs have been the most important food source. We examined the allergenic properties of eggs from varying birds on patients with known allergy to chick-en eggs, to find out whether they can replace the chicken eggs for the source of nutrient. Methods : Samples were selected. from patie"Its who visited the allergy clinic of the Department of Pediatrics at Yonsei University Medcal Center. The serum specific IgE for eggs were measured and allergy skin tests were performed. The serum of the patients with proven egg allergy was then tested for reactivity with eggs from wild and domestic ducks, geese, seagulls, quails, yellow-shelled and white-shelled eggs employing SDS--PAGE and IgE imrnunoblotting. Results : All the egg protein shr)wed similar molecular sizes ranging from 24-100 kDa. Their expressions, however, were different, with white eggs, yellow eggs, and quail eggs showing strong reaction, while ducks, geese, and mallard eggs presented with weak reaction. Imrnunoblotting exhibited reactivity in 35-50 kDa and 25-35 kDa groups. Quail eggs and shells from chicken eggs showed a protein banding of 75-80 kDa. Geese, wild and domestic ducks, quail did not exhibit any cross-reactivity with chicken eggs. Conclusion :Among patients with egg allergy, cross-reactivity between different chicken eggs was present, but no cross-reactivity was apparent between chicken eggs and other birds´ eggs. Therefore, we suggest theses eggs as alternative source of food in patients with egg allergy. However further study with larger patient population is still required.