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사람 코점막과 배양된 코점막 상피세포에서 15-Lipoxygenase와 Cyclooxygenase-2의 발현

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dc.contributor.author김경수-
dc.contributor.author김창훈-
dc.contributor.author윤주헌-
dc.date.accessioned2015-07-15T17:00:48Z-
dc.date.available2015-07-15T17:00:48Z-
dc.date.issued2003-
dc.identifier.issn1225-035X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/114051-
dc.description.abstractBackground and Objectives : The purpose of our study was to examine the expression of 15-lipoxygenase (15-LO) in human nasal mucosa and to investigate the change of expression of 15-LO and eyelooxygenase-2 (COX-2) as a funtion of mucociliary and squamous differenciation. Materials and Method : Human inferior turbinates were used and immunohistochemistry with 15-LO and COX-2 antibody was done. passage-2 normal human nasal epithelial cell culture using air-liquid interface method was performed for 14 days and the cells were divided as retinoic acid (RA)-sufficient and RA-deficient group. Western blot analysis for 15-LO and COX-2 expression was performed on each group on 0, 7, and 14 days. Results : 15-LO expression of mucociliated epithelium was noted in ciliated cells and basal cells, but was not found in goblet cells and secretory acini. In squamous epithelium, the expression of 15-LO was weaker than that in the mucociliated epithelium, but the expression of COX-2 showed no difference between them. In Western blot analysis, 15-LO expression was significantly higher in RA-sufficient culture than in RA-deficient culture and this expression was time-dependent. COX-2 expression was almost same level in RA-deficient culture, but its expression was significantly higher in RA-sufficient culture on 7 and 14 days than zero day. Conclusion : 15-LO and COX-2 may be related to differentiation and development of nasal epithelial cells. However, it is nuclear whether this relationship is direct or indirect effect of 15-LO and COX-2. This question remains to be solved.-
dc.description.statementOfResponsibilityopen-
dc.format.extent649~653-
dc.relation.isPartOfKorean Journal of Otolaryngology (대한이비인후과학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHNasal mucosa-
dc.subject.MESH15-Lipoxygenase, arachidonate-
dc.subject.MESHSynthase, prostaglandin-
dc.subject.MESHCell differentiation-
dc.title사람 코점막과 배양된 코점막 상피세포에서 15-Lipoxygenase와 Cyclooxygenase-2의 발현-
dc.title.alternativeExpression of 15-Lipoxygenase and Cyclooxygen-2 in Human Nasal Mucosa and Cultured Human Nasal Epithelial Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학)-
dc.contributor.googleauthor김경수-
dc.contributor.googleauthor윤주헌-
dc.contributor.googleauthor김지훈-
dc.contributor.googleauthor장정현-
dc.contributor.googleauthor김선구-
dc.contributor.googleauthor김창훈-
dc.identifier.doiOAK-2003-00891-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01050-
dc.contributor.localIdA00298-
dc.contributor.localIdA02604-
dc.relation.journalcodeJ02088-
dc.subject.keywordNasal mucosa-
dc.subject.keyword15-Lipoxygenase, arachidonate-
dc.subject.keywordSynthase, prostaglandin-
dc.subject.keywordCell differentiation-
dc.contributor.alternativeNameKim, Kyung Su-
dc.contributor.alternativeNameKim, Chang Hoon-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.affiliatedAuthorKim, Chang Hoon-
dc.contributor.affiliatedAuthorKim, Kyung Su-
dc.contributor.affiliatedAuthorYoon, Joo Heon-
dc.rights.accessRightsfree-
dc.citation.volume46-
dc.citation.number8-
dc.citation.startPage649-
dc.citation.endPage653-
dc.identifier.bibliographicCitationKorean Journal of Otolaryngology (대한이비인후과학회지), Vol.46(8) : 649-653, 2003-
dc.identifier.rimsid55679-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

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