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사람 코점막과 배양된 코점막 상피세포에서 15-Lipoxygenase와 Cyclooxygenase-2의 발현
DC Field | Value | Language |
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dc.contributor.author | 김경수 | - |
dc.contributor.author | 김창훈 | - |
dc.contributor.author | 윤주헌 | - |
dc.date.accessioned | 2015-07-15T17:00:48Z | - |
dc.date.available | 2015-07-15T17:00:48Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1225-035X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114051 | - |
dc.description.abstract | Background and Objectives : The purpose of our study was to examine the expression of 15-lipoxygenase (15-LO) in human nasal mucosa and to investigate the change of expression of 15-LO and eyelooxygenase-2 (COX-2) as a funtion of mucociliary and squamous differenciation. Materials and Method : Human inferior turbinates were used and immunohistochemistry with 15-LO and COX-2 antibody was done. passage-2 normal human nasal epithelial cell culture using air-liquid interface method was performed for 14 days and the cells were divided as retinoic acid (RA)-sufficient and RA-deficient group. Western blot analysis for 15-LO and COX-2 expression was performed on each group on 0, 7, and 14 days. Results : 15-LO expression of mucociliated epithelium was noted in ciliated cells and basal cells, but was not found in goblet cells and secretory acini. In squamous epithelium, the expression of 15-LO was weaker than that in the mucociliated epithelium, but the expression of COX-2 showed no difference between them. In Western blot analysis, 15-LO expression was significantly higher in RA-sufficient culture than in RA-deficient culture and this expression was time-dependent. COX-2 expression was almost same level in RA-deficient culture, but its expression was significantly higher in RA-sufficient culture on 7 and 14 days than zero day. Conclusion : 15-LO and COX-2 may be related to differentiation and development of nasal epithelial cells. However, it is nuclear whether this relationship is direct or indirect effect of 15-LO and COX-2. This question remains to be solved. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Korean Journal of Otolaryngology (대한이비인후과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Nasal mucosa | - |
dc.subject.MESH | 15-Lipoxygenase, arachidonate | - |
dc.subject.MESH | Synthase, prostaglandin | - |
dc.subject.MESH | Cell differentiation | - |
dc.title | 사람 코점막과 배양된 코점막 상피세포에서 15-Lipoxygenase와 Cyclooxygenase-2의 발현 | - |
dc.title.alternative | Expression of 15-Lipoxygenase and Cyclooxygen-2 in Human Nasal Mucosa and Cultured Human Nasal Epithelial Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | 김경수 | - |
dc.contributor.googleauthor | 윤주헌 | - |
dc.contributor.googleauthor | 김지훈 | - |
dc.contributor.googleauthor | 장정현 | - |
dc.contributor.googleauthor | 김선구 | - |
dc.contributor.googleauthor | 김창훈 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01050 | - |
dc.contributor.localId | A00298 | - |
dc.contributor.localId | A02604 | - |
dc.relation.journalcode | J02088 | - |
dc.subject.keyword | Nasal mucosa | - |
dc.subject.keyword | 15-Lipoxygenase, arachidonate | - |
dc.subject.keyword | Synthase, prostaglandin | - |
dc.subject.keyword | Cell differentiation | - |
dc.contributor.alternativeName | Kim, Kyung Su | - |
dc.contributor.alternativeName | Kim, Chang Hoon | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Kim, Chang Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Su | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 46 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 649 | - |
dc.citation.endPage | 653 | - |
dc.identifier.bibliographicCitation | Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.46(8) : 649-653, 2003 | - |
dc.identifier.rimsid | 55679 | - |
dc.type.rims | ART | - |
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