Cited 13 times in
Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory–secretory antigens
DC Field | Value | Language |
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dc.contributor.author | 용태순 | - |
dc.date.accessioned | 2015-07-15T16:57:58Z | - |
dc.date.available | 2015-07-15T16:57:58Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0932-0113 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113955 | - |
dc.description.abstract | Clonorchis sinensis excretory–secretory (ES) antigens were separated by gradient SDS-PAGE and the antigenic protein isolated at 21 kDa was injected into rats. A C. sinensis cDNA library was then immunoscreened with sera from the 21-kDa antigenic protein-immunized rats. The selected genes, which were named C. sinensis myoglobin, contained a single open reading frame of 450 base pairs encoding 150 amino acids. A single hybridized band of 0.57 kb and two to four hybridized bands were detected by Northern and Southern blotting. Purified recombinant C. sinensis myoglobin was recognized by clonorchiasic rabbit sera (50%) and clonorchiasic human sera (25%). An indirect immunofluorescence assay showed that C. sinensis myoglobin is distributed over the whole body of the adult worm. It is believed that the abundance of C. sinensis myoglobin plays an important role as an oxygen reservoir under anaerobic conditions. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 338~343 | - |
dc.relation.isPartOf | PARASITOLOGY RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, Helminth*/immunology | - |
dc.subject.MESH | Antigens, Helminth*/isolation & purification | - |
dc.subject.MESH | Blotting, Northern | - |
dc.subject.MESH | Blotting, Southern | - |
dc.subject.MESH | Cloning, Molecular* | - |
dc.subject.MESH | Clonorchis sinensis/immunology* | - |
dc.subject.MESH | Clonorchis sinensis/metabolism | - |
dc.subject.MESH | Fluorescent Antibody Technique, Indirect | - |
dc.subject.MESH | Gene Library | - |
dc.subject.MESH | Helminth Proteins/genetics* | - |
dc.subject.MESH | Helminth Proteins/immunology | - |
dc.subject.MESH | Helminth Proteins/isolation & purification | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Myoglobin/genetics* | - |
dc.subject.MESH | Myoglobin/immunology | - |
dc.subject.MESH | Myoglobin/isolation & purification | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Recombinant Proteins/analysis | - |
dc.subject.MESH | Recombinant Proteins/immunology | - |
dc.subject.MESH | Recombinant Proteins/metabolism | - |
dc.title | Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory–secretory antigens | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Environmental Medical Biology (환경의생물학) | - |
dc.contributor.googleauthor | Seobo Sim | - |
dc.contributor.googleauthor | Gab-Man Park | - |
dc.contributor.googleauthor | Tai-Soon Yong | - |
dc.identifier.doi | 10.1007/s00436-003-0869-7 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02424 | - |
dc.relation.journalcode | J02467 | - |
dc.identifier.eissn | 1432-1955 | - |
dc.identifier.pmid | 14574567 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs00436-003-0869-7 | - |
dc.subject.keyword | Adult Worm | - |
dc.subject.keyword | Recombinant Fusion Protein | - |
dc.subject.keyword | Indirect Immunofluorescence Assay | - |
dc.subject.keyword | Single Open Reading Frame | - |
dc.subject.keyword | Reactive Band | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 91 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 338 | - |
dc.citation.endPage | 343 | - |
dc.identifier.bibliographicCitation | PARASITOLOGY RESEARCH, Vol.91(4) : 338-343, 2003 | - |
dc.identifier.rimsid | 54014 | - |
dc.type.rims | ART | - |
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