Cited 0 times in
아토피피부염 환자에서 인터루킨-12에 의한 CD4+ 세포 단백 변화에 관한 프로테옴 분석
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이광훈 | - |
dc.date.accessioned | 2015-07-15T16:57:01Z | - |
dc.date.available | 2015-07-15T16:57:01Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1225-8180 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113923 | - |
dc.description.abstract | The pathogenesis of atopic dermatitis is based on an inflammatory mechanism involving type 2 cytokines such as interleukin (IL)-4 and IL-13. CD4+ T cells in atopic dermatitis express predominantly T helper (Th)2 phenotype and they down-regulates IL-12Rβ2. IL-12 is a major cytokine in the differentiation of naive CD4+ T cells into Th1 cells. This mechanism is closely related with the expression of IL-12Rβ2. However, it remains unclear that IL-12 signaling mechanism in polarized Th2 cells. The aim of this study was to identify IL-12 responsiveness in CD4+ T cells of patients with atopic dermatitis using a proteomic tools. CD4+ T cells isolated from peripheral blood of patients with atopic dermatitis were treated with neutralizing anti-IL-4 antibody (200 ng/ml) and IL-12 (2 ng/ml) and parallel cultures of untreated cells were also prepared. On day 3, surface phenotype change was examined using IL-12Rβ2 antibody by FACS analysis and separate CD4+ T cells by two-dimensional electrophoresis (2-DE). About 1500 protein spots were detected in the 2-DE gels by modified silver staining. Several areas of the 2-DE map exhibited quantitative and qualitative changes after IL-12 treatment. Several regions including actins showed variations according to the samples. A group of three spots detected in the area of pI 6.0-10.0 with molecular weight about 40 kDa. In addition, decreased or increased spots were observed in the regions of pI 6.5-7.0 with molecular weight about 20 kDa. The identification of these spots must be established in further study in order to find targets regulated by IL-12 in CD4+ T cells of atopic patients. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Korean Journal of InvestigativeDermatology (대한피부연구학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Atopic dermatitis | - |
dc.subject.MESH | proteomics | - |
dc.subject.MESH | CD4+ T cells | - |
dc.subject.MESH | Interleukin-12 | - |
dc.title | 아토피피부염 환자에서 인터루킨-12에 의한 CD4+ 세포 단백 변화에 관한 프로테옴 분석 | - |
dc.title.alternative | Proteomic Approach to CD4+ T Cell Differentiation Protein by Interleukin -12 in Patients with Atopic Dermatitis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학) | - |
dc.contributor.googleauthor | 이효연 | - |
dc.contributor.googleauthor | 이주희 | - |
dc.contributor.googleauthor | 이호연 | - |
dc.contributor.googleauthor | 이광훈 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02674 | - |
dc.relation.journalcode | J02042 | - |
dc.subject.keyword | Atopic dermatitis | - |
dc.subject.keyword | proteomics | - |
dc.subject.keyword | CD4+ T cells | - |
dc.subject.keyword | Interleukin-12 | - |
dc.contributor.alternativeName | Lee, Kwang Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Kwang Hoon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 10 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 127 | - |
dc.citation.endPage | 135 | - |
dc.identifier.bibliographicCitation | Korean Journal of InvestigativeDermatology (대한피부연구학회지), Vol.10(3) : 127-135, 2003 | - |
dc.identifier.rimsid | 53987 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.