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Bone Morphogenetic Protein-2 Facilitates Expression of Chondrogenic, not Osteogenic, Phenotype of Human Intervertebral Disc Cells
DC Field | Value | Language |
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dc.contributor.author | 문성환 | - |
dc.contributor.author | 한수봉 | - |
dc.date.accessioned | 2015-07-15T16:51:45Z | - |
dc.date.available | 2015-07-15T16:51:45Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0362-2436 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113745 | - |
dc.description.abstract | STUDY DESIGN: In vitro experiment using bone morphogenetic protein-2 (BMP-2) and human intervertebral disc (IVD) cells. OBJECTIVES: To demonstrate the effect of BMP-2 on mRNAs expression (collagen type I, collagen type II, aggrecan, and osteocalcin), proteoglycan synthesis, expression of alkaline phosphatase, bone nodule formation in human IVD cells. SUMMARY OF BACKGROUND DATA: BMP-2 was widely known as a powerful agent for osteoinduction and a crucial growth factor for early chondrogenesis and maintenance of cartilaginous phenotype. BMP-2 proved to be effective in stimulating proteoglycan synthesis in articular chondrocytes and IVD cells. Nevertheless, the effect of BMP-2 on IVD cells, whether chondrogenic or osteogenic, was not thoroughly elucidated in transcriptional level and histochemical stains. MATERIALS AND METHODS: Human IVDs were harvested and enzymatically digested. Then IVD cells were cultured three-dimensionally in alginate beads. Osteoblasts were cultured from cancellous bone of ilium for histochemical stains. Recombinant human BMP-2 (rhBMP-2) was produced by Chinese hamster ovary cells after transduction of BMP-2 cDNA, then concentrated and purified. Then IVD cell cultures were exposed to various concentrations of rhBMP-2. Reverse transcription-polymerase chain reaction for mRNA expression of aggrecan, collagen type I, collagen type II, and osteocalcin was performed. Newly synthesized proteoglycan was measured by 35S-sulfate incorporation on Sephadex G-25 M in PD 10 columns. As a histochemical examination, alkaline phosphatase and Alizarin red-S stains were used to detect osteogenic marker and bone nodule formation, respectively. RESULTS: In the rhBMP-2 treated cultures, there was increased newly synthesized proteoglycan (67% in 300 ng/mL and 200% in 1,500 ng/mL of rhBMP-2) and up-regulated expression of aggrecan, collagen type I, and collagen type II mRNA over untreated control. However, rhBMP-2 did not up-regulate expression of osteocalcin mRNA in the given dose and culture period. IVD cell cultures with rhBMP-2 showed no evidence of bone formation in histochemical stains, i.e., alkaline phosphatase and Alizarin red-S, while osteoblast culture exhibited strong positive stains. CONCLUSIONS: The rhBMP-2 clearly up-regulated mRNA expression of chondrogenic components and also stimulated proteoglycan synthesis without expression of osteogenic phenotype. Taken together, this study raise the possibility of rhBMP-2 can be anabolic agent for regenerating matrix of intervertebral disc. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | SPINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Bone Morphogenetic Protein-2 Facilitates Expression of Chondrogenic, not Osteogenic, Phenotype of Human Intervertebral Disc Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | Dong-Jun Kim | - |
dc.contributor.googleauthor | Seong-Hwan Moon | - |
dc.contributor.googleauthor | Hwan-Mo Lee | - |
dc.contributor.googleauthor | Soo-Bong Hahn | - |
dc.contributor.googleauthor | Keong-Jin Han | - |
dc.contributor.googleauthor | Moon-Soo Park | - |
dc.contributor.googleauthor | Un-Hye Kwon | - |
dc.contributor.googleauthor | Hyang Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01365 | - |
dc.contributor.localId | A04293 | - |
dc.relation.journalcode | J02674 | - |
dc.identifier.eissn | 1528-1159 | - |
dc.identifier.url | http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00007632-200312150-00010&LSLINK=80&D=ovft | - |
dc.contributor.alternativeName | Moon, Seong Hwan | - |
dc.contributor.alternativeName | Hahn, Soo Bong | - |
dc.contributor.affiliatedAuthor | Moon, Seong Hwan | - |
dc.contributor.affiliatedAuthor | Hahn, Soo Bong | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 28 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 2679 | - |
dc.citation.endPage | 2684 | - |
dc.identifier.bibliographicCitation | SPINE, Vol.28(24) : 2679-2684, 2003 | - |
dc.identifier.rimsid | 49062 | - |
dc.type.rims | ART | - |
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