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Bone Morphogenetic Protein-2 Facilitates Expression of Chondrogenic, not Osteogenic, Phenotype of Human Intervertebral Disc Cells

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dc.contributor.author문성환-
dc.contributor.author한수봉-
dc.date.accessioned2015-07-15T16:51:45Z-
dc.date.available2015-07-15T16:51:45Z-
dc.date.issued2003-
dc.identifier.issn0362-2436-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/113745-
dc.description.abstractSTUDY DESIGN: In vitro experiment using bone morphogenetic protein-2 (BMP-2) and human intervertebral disc (IVD) cells. OBJECTIVES: To demonstrate the effect of BMP-2 on mRNAs expression (collagen type I, collagen type II, aggrecan, and osteocalcin), proteoglycan synthesis, expression of alkaline phosphatase, bone nodule formation in human IVD cells. SUMMARY OF BACKGROUND DATA: BMP-2 was widely known as a powerful agent for osteoinduction and a crucial growth factor for early chondrogenesis and maintenance of cartilaginous phenotype. BMP-2 proved to be effective in stimulating proteoglycan synthesis in articular chondrocytes and IVD cells. Nevertheless, the effect of BMP-2 on IVD cells, whether chondrogenic or osteogenic, was not thoroughly elucidated in transcriptional level and histochemical stains. MATERIALS AND METHODS: Human IVDs were harvested and enzymatically digested. Then IVD cells were cultured three-dimensionally in alginate beads. Osteoblasts were cultured from cancellous bone of ilium for histochemical stains. Recombinant human BMP-2 (rhBMP-2) was produced by Chinese hamster ovary cells after transduction of BMP-2 cDNA, then concentrated and purified. Then IVD cell cultures were exposed to various concentrations of rhBMP-2. Reverse transcription-polymerase chain reaction for mRNA expression of aggrecan, collagen type I, collagen type II, and osteocalcin was performed. Newly synthesized proteoglycan was measured by 35S-sulfate incorporation on Sephadex G-25 M in PD 10 columns. As a histochemical examination, alkaline phosphatase and Alizarin red-S stains were used to detect osteogenic marker and bone nodule formation, respectively. RESULTS: In the rhBMP-2 treated cultures, there was increased newly synthesized proteoglycan (67% in 300 ng/mL and 200% in 1,500 ng/mL of rhBMP-2) and up-regulated expression of aggrecan, collagen type I, and collagen type II mRNA over untreated control. However, rhBMP-2 did not up-regulate expression of osteocalcin mRNA in the given dose and culture period. IVD cell cultures with rhBMP-2 showed no evidence of bone formation in histochemical stains, i.e., alkaline phosphatase and Alizarin red-S, while osteoblast culture exhibited strong positive stains. CONCLUSIONS: The rhBMP-2 clearly up-regulated mRNA expression of chondrogenic components and also stimulated proteoglycan synthesis without expression of osteogenic phenotype. Taken together, this study raise the possibility of rhBMP-2 can be anabolic agent for regenerating matrix of intervertebral disc.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfSPINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleBone Morphogenetic Protein-2 Facilitates Expression of Chondrogenic, not Osteogenic, Phenotype of Human Intervertebral Disc Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Orthopedic Surgery (정형외과학)-
dc.contributor.googleauthorDong-Jun Kim-
dc.contributor.googleauthorSeong-Hwan Moon-
dc.contributor.googleauthorHwan-Mo Lee-
dc.contributor.googleauthorSoo-Bong Hahn-
dc.contributor.googleauthorKeong-Jin Han-
dc.contributor.googleauthorMoon-Soo Park-
dc.contributor.googleauthorUn-Hye Kwon-
dc.contributor.googleauthorHyang Kim-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01365-
dc.contributor.localIdA04293-
dc.relation.journalcodeJ02674-
dc.identifier.eissn1528-1159-
dc.identifier.urlhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00007632-200312150-00010&LSLINK=80&D=ovft-
dc.contributor.alternativeNameMoon, Seong Hwan-
dc.contributor.alternativeNameHahn, Soo Bong-
dc.contributor.affiliatedAuthorMoon, Seong Hwan-
dc.contributor.affiliatedAuthorHahn, Soo Bong-
dc.rights.accessRightsnot free-
dc.citation.volume28-
dc.citation.number24-
dc.citation.startPage2679-
dc.citation.endPage2684-
dc.identifier.bibliographicCitationSPINE, Vol.28(24) : 2679-2684, 2003-
dc.identifier.rimsid49062-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers

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