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정상 유방 조직과 유방암 조직에서 에스트로겐 수용체-베타 및 변형체 발현의 의의
DC Field | Value | Language |
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dc.contributor.author | 박병우 | - |
dc.date.accessioned | 2015-07-15T16:48:12Z | - |
dc.date.available | 2015-07-15T16:48:12Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1738-6756 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113629 | - |
dc.description.abstract | Purpose : Since the discovery of estrogen receptor-β (ER-β), five C-terminal variants of ER-β were identified. We designed this study to investigate the pattern and clinical implications of ER-β and its splicing variants expression in normal and malignant mammary tissues. Methods : Using reverse transcription polymerase chain reaction (RT-PCR), we examined the expression levels of ER-α and ER-β and its five splicing variants (β1, β2, β3, β4, β5) in 50 paired normal and cancer tissues. We measured the densities of RT-PCR products using Tina version 2.10 (Raytest, Germany). Firstly, the incidence and intensity of ER-α and ER-β and its five splicing variants were compared. Then the expression of ER-β mRNA splicing variants was also analyzed with regard to the ER-α protein expression measured by immuno-histochemical staining and the menopausal status of the patients. Chi-square test and paired samples t-test were used for statistical analysis. Differences were considered to be significant with a p-value of less than 0.05. Results : The expression of ER-β mRNA variants in normal breast and cancer tissues were as follows: ER-β2 (100%/100%), ER-β4 (76%/74%), ER-β5 (32%/58%), and ER-β1 (14%/16%). ER-β3 was not detected at all. In terms of intensity, we observed a significant decrease of ER-β2 (P<0.001) and an increase of ER-β5 (P=0.004) in the mRNA expression levels among breast cancers compared to the correstponding normal breast tissues. Compared to the corresponding normal tissues, a significant decrease of ER-β2 in cancer tissues was observed in patients with ER-α-positive (P<0.001), with age over 50 (P=0.01), and under 50 (P=0.04) as well, but not in patients with ER-α-negative (P=0.48). ER-β4 also significantly decreased in paitents with ER-α-positive (P=0.004) and with age over 50 (P=0.07). ER-β5 showed a significant increment only in patient aged over 50 (P=0.04). Conclusion : ER-α mRNA expression significantly increases but ER-β mRNA expression decreases in the cancer tissues compared to the corresponding normal tissues. Among ER-β variant forms, ER-β2 is predominant in both normal and malignant mammary tissues and ER-β4, ER-β5, and ER-β1 in descending order but ER-β3 does not express in mammary tissues. The decrease of ER-β2 and ER-β4 expression is prominent in cancer tissue especially in ER-α-positive cancers, which suggests that ER-β2 and ER-β4 may possess a regulatory function in mammary carcinogenesis. Further investigations to verify the roles of ER-β variants are mandatory. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Journal of Korean Breast Cancer Society (한국유방암학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | breast cancer | - |
dc.subject.MESH | estrogen receptors | - |
dc.subject.MESH | ER-β variant | - |
dc.subject.MESH | carcinogenesis | - |
dc.title | 정상 유방 조직과 유방암 조직에서 에스트로겐 수용체-베타 및 변형체 발현의 의의 | - |
dc.title.alternative | Expression of Estrogen Receptor-β and Its Variants in Normal Mammary and Tumor Tissues | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | 김기석 | - |
dc.contributor.googleauthor | 허민규 | - |
dc.contributor.googleauthor | 박병우 | - |
dc.contributor.googleauthor | 김승일 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01475 | - |
dc.relation.journalcode | J01504 | - |
dc.subject.keyword | breast cancer | - |
dc.subject.keyword | estrogen receptors | - |
dc.subject.keyword | ER-β variant | - |
dc.subject.keyword | carcinogenesis | - |
dc.contributor.alternativeName | Park, Byeong Woo | - |
dc.contributor.affiliatedAuthor | Park, Byeong Woo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 6 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 161 | - |
dc.citation.endPage | 167 | - |
dc.identifier.bibliographicCitation | Journal of Korean Breast Cancer Society (한국유방암학회지), Vol.6(2) : 161-167, 2003 | - |
dc.identifier.rimsid | 44798 | - |
dc.type.rims | ART | - |
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