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척추 골유합에 있어 황색 인대의 이중 역할: 골형성 유도 기능 및 체외 유전자 전달 매개체
DC Field | Value | Language |
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dc.contributor.author | 문성환 | - |
dc.date.accessioned | 2015-07-15T16:44:43Z | - |
dc.date.available | 2015-07-15T16:44:43Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 2093-4378 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113513 | - |
dc.description.abstract | STUDY DESIGN: An in-vitro experiment using human ligamentum flavum (LF) and the adnovirus-BMP-2 construct, Ad/BMP-2. OBJECTIVES: To determine the dual roles of LF as an osteoinductive agent and carrier for ex-vivo gene transfer. SUMMARY OF LITERATURE REVIEW: LF is known to have osteogenic potential. Pathologically, ossified LF may cause myelopathy and radiculopathy. BMP-2 is known as an important factor in the differentiation, and maintenance, of osteoblast phenotypes. Ex-vivo gene transfer, using human LF for spinal fusion, has never been attempted before. MATERIALS AND METHODS: The LF cells were cultured from the degenerated LF of spinal stenosis patients. An adenovirus construct, containing BMP-2 cDNA (Ad/BMP-2), was also produced. The LF cell cultures were exposed to the adenoviral construct. The Osteocalcin expression was analysed by Western blot analysis. The osteocalcin and BMP-2 mRNA expressions were analysed by RT-PCR. Bone formation was assessed by alkaline phosphatase and Von Kossa stains. RESULTS: The LF cell cultures, with Ad/BMP-2, showed transgene expression in the Western blot analysis. Also, the cultures exhibited the mRNA expressions of both osteocalcin and BMP-2, in a dose-dependent manner. The LF cultures, with Ad/BMP-2, demonstrated alkaline phosphatase expression and bone nodule formations from the Von Kossa staining. CONCLUSION: The genetically modified LF strongly induced osteogenesis, which can be used during a spinal fusion, as an osteoinductive agent and carrier, for ex-vivo gene transfer. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1~7 | - |
dc.relation.isPartOf | Journal of Korean Society of Spine Surgery (대한척추외과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Ligamentum flavum | - |
dc.subject.MESH | BMP-2 | - |
dc.subject.MESH | Adenovirus | - |
dc.subject.MESH | Gene transfer | - |
dc.title | 척추 골유합에 있어 황색 인대의 이중 역할: 골형성 유도 기능 및 체외 유전자 전달 매개체 | - |
dc.title.alternative | Dual Roles of Ligamentum Flavum for Spinal Fusion: As an Osteoinductive Agent and Carrier for Ex-vivo Gene Transfer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Orthopedic Surgery (정형외과학) | - |
dc.contributor.googleauthor | 문성환 | - |
dc.contributor.googleauthor | 김향 | - |
dc.contributor.googleauthor | 이환모 | - |
dc.contributor.googleauthor | 한수봉 | - |
dc.contributor.googleauthor | 김학선 | - |
dc.contributor.googleauthor | 윤홍기 | - |
dc.contributor.googleauthor | 김경희 | - |
dc.contributor.googleauthor | 권언혜 | - |
dc.identifier.doi | 10.4184/jkss.2003.10.1.1 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01365 | - |
dc.relation.journalcode | J01550 | - |
dc.identifier.pmid | 10.4184/jkss.2003.10.1.1 | - |
dc.subject.keyword | Ligamentum flavum | - |
dc.subject.keyword | BMP-2 | - |
dc.subject.keyword | Adenovirus | - |
dc.subject.keyword | Gene transfer | - |
dc.contributor.alternativeName | Moon, Seong Hwan | - |
dc.contributor.affiliatedAuthor | Moon, Seong Hwan | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 7 | - |
dc.identifier.bibliographicCitation | Journal of Korean Society of Spine Surgery (대한척추외과학회지), Vol.10(1) : 1-7, 2003 | - |
dc.identifier.rimsid | 52177 | - |
dc.type.rims | ART | - |
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