Rifampin내성결핵의 조기 진단을 위한 RNA/RNA duplex, base pair-mismatch assay와 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay 의 유용성

Abstract

Background:Multidrug-resistant tuberculosis is an increasing concern for public health in many parts of the world. We have evaluated the specificity and sensitivity of the mismatch assay and 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in detecting rifampin- resistant Mycobacterium tuberculosis. Methods:Eleven rifampin-susceptible and 15 rifampin-resistant M. tuberculosis strains were isolated from clinical specimens obtained from patients in Yonsei University College of Medicine, Severance Hospital. RNA/RNA duplex, base pair-mismatch assay (Mismatch Detect II kit, Ambion) and MTT assay were performed. Results:The specificity and sensitivity of detection of rifampin resistance were 91% and 87% in mismatch assay and 73% and 67% in MTT assay, respectively. Conclusion:These results suggest the usefulness of mismatch assay in detecting rifampin-resistant Mycobacterium tuberculosis.